中国麻风皮肤病杂志 ›› 2018, Vol. 34 ›› Issue (6): 324-326.

• 论著 • 上一篇    下一篇

miR-34a-5p对HaCaT细胞增殖及其靶基因Notch1表达的影响

朱杰1，王慧琴1，赵宗峰1，张慧1，康晓静2，吴卫东1   

  1. 1新疆维吾尔自治区人民医院临床医学研究中心，新疆 乌鲁木齐 830001 2新疆维吾尔自治区人民医院皮肤科，新疆 乌鲁木齐830001
  • 出版日期:2018-06-15 发布日期:2018-12-11
  • 通讯作者: 吴卫东，E-mail: xjwudong@126.com
  • 基金资助:
    新疆维吾尔自治区自然科学基金（编号：201521C201）

Effects of miR-34a-5p on the proliferation and expressions of Notch1 in HaCaT Cells

ZHU Jie1, WANG Huiqin1, ZHAO Zongfeng1, ZHAGN Hui 1, KANG Xiaojing2, WU Weidong1   

  1. 1．People’s Hospital of Xinjiang Uygur Autonomous Region，the Center of Clinical Research，Urumqi 830001，China；2．People’s Hospital of Xinjiang Uygur Autonomous Region，Department of Dermatology and Venereology，Urumqi 830001，China
  • Online:2018-06-15 Published:2018-12-11
  • Contact: WU Weidong，E-mail：xjwudong@126.com

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摘要： 目的: 检测miR-34a-5p对人角质形成细胞株HaCaT细胞体外增殖及其靶基因Notch1 mRNA表达的影响。方法: HaCaT细胞体外培养，优化转染浓度,分为4组进行转染：miR-34a-5p 模拟物组（minic 组），阴性对照组（NC组），miR-34a-5p 抑制物组（inhibitor组），抑制物阴性对照组（inhibitor NC组）。MTT检测细胞增殖情况； RT-qPCR检测Notch1的mRNA表达情况。结果: 转染后48小时minic 组、inhibitor组细胞增殖活力分别为39.7%和15.7%，minic 组、inhibitor组与NC组（21.3%）相比，差异有统计学意义(Ps<0.05)。HaCaT细胞转染后，minic 组和inhibitor组Notch1表达量分别为2.78±1.30和0.37±0.46，与NC组(1.0±0.06)比较，差异均有统计学意义(P<0.05)。结论: miR-34a-5p促进HaCaT细胞体外增殖，机制可能与Notch1 mRNA表达上调有关。

关键词: 银屑病, miR-34a, HaCaT细胞, notch1

Abstract: Objective: To determine the effects of miR-34a-5p on proliferation of HaCaT human keratinocytes and expressions of Notch1 mRNA. Methods: HaCaT cells were cultured in vitro and transfection concentration were optimized. And than, the cells were divided into 4 groups: miR-34a-5p minic group (minic), negative control group (NC)，miR-34a-5p inhibitor group (inhibitor) and inhibitor negative control group (inhibitor NC). Cell proliferation was detected by MTT assay. The expression of Notch1 mRNA was detected by RT-qPCR. Results: After transfected for 48 hours, the proliferation vitality of HaCaT cells and the level of Notch1 in NC group were 20.7% and 1.0 ± 0.06 respectively, those in minic group were 39.7% and 2.78 ± 1.30, those in inhibitor group was 15.7% and 0.37 ± 0.46. There were significant differences in the proliferation vitality of HaCaT cells and the level of Notch1 between minic group, inhibitor group and NC group (Ps＜0.05). Conclusion: miR-34a can promote the proliferation of HaCaT cells, which may be related to the upregulation of Notch1 mRNA.

Key words: psoriasis, miR-34a, HaCaT cell, Notch1