中国麻风皮肤病杂志 ›› 2016, Vol. 32 ›› Issue (11): 648-652.

• 论著 • 上一篇    下一篇

TL1A及其受体DcR3在寻常型银屑病皮损中的表达

张丽芳，盛晚香，缪泽群，宋继权   

  1. 武汉大学中南医院皮肤科，湖北武汉,430071
  • 出版日期:2016-11-15 发布日期:2018-12-19
  • 通讯作者: 盛晚香，E-mail: shengwanxiang@126.com

Expression of TL1A and its receptorDcR3 in the skin lesions of psoriasis vulgaris

ZHANG Lifang，SHENG Wanxiang，MIAO Zequn，SONG Jiquan   

  1. Department of Dermatology，Zhongnan Hospital，Wuhan University，Wuhan 430071，China
  • Online:2016-11-15 Published:2018-12-19
  • Contact: SHENG Wanxiang, E-mail: shengwanxiang@126.com

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摘要： 目的：检测TL1A及其受体DcR3在寻常型银屑病患者皮损组织中的表达。方法：采用免疫组织化学法和实时荧光定量RT-PCR法对30例寻常型银屑病患者皮损中TL1A和DcR3水平进行检测。结果：银屑病组中TL1A和DcR3蛋白阳性表达率分别为93.3%和90%，正常对照组中TL1A和DcR3蛋白阳性表达率分别为20%和60%；银屑病组TL1AmRNA和DcR3 mRNA表达CT值分别为4.909土1.607和3.430土1.449，正常对照组TL1AmRNA和DcR3 mRNA表达CT值分别为1.250土0.650和1.011土0.150，银屑病组中TL1A和DcR3蛋白及mRNA水平明显高于正常对照，差异均有统计学意义。银屑病皮损中TL1A与DcR3的表达以及TL1AmRNA与DcR3mRNA蛋白的表达均呈正相关性（r=0.735 P<0.01和r=0.2436 P>0.05）。结论：TL1A和DcR3可能参与银屑病炎症的发生机制。

关键词: 寻常型银屑病, 肿瘤坏死因子样配体1A, 诱骗受体3

Abstract: Objective：To investigate the expression of TNF-like ligand 1 aberrance (TL1A) and its receptor decoy receptor3 (DcR3) in the skin lesions of the patients with psoriasis vulgaris (PV).. Methods：The expression of TL1A and DcR3 in the skin lesions of 30 patients with PV was tested with SP immunohistochemical and real-time fluorescence quantitative RT-PCR method. Results: The positivity rates of TL1A and DcR3 proteins were 93.3% and 90% in PV, and were 20% and 60% in the normal samples. The expression levels of TL1A mRNA and DcR3 mRNA were (4.909土1.607) and (3.430土1.449) in PV, and (1.250土0.650) and (1.011土0.150) in normal skin. The level of TL1A and DcR3 protein and mRNA were significantly higher in PV than that in normal tissue, with a significant difference.?There was a positively correlation?between the protein level of TL1A and DcR3 in PS（P<0.01）; TL1AmRNA and DCR3mRNA expression in psoriatic lesion was a positive correlation (P<0.05); TL1A mRNA expression in PS and PASI shows no correlation (P>0.05); DcR3 mRNA expression in PS was positively correlation with PASI (P<0.05). Conclusion：TL1A and DcR3 may participate in the pathogenesis of chronic skin inflammation in psoriasis.

Key words: psoriasis vulgaris, TNF-like ligand 1 aberrance, decoy receptor3