中国麻风皮肤病杂志 ›› 2020, Vol. 36 ›› Issue (6): 330-334.doi: 10.12144/zgmfskin202006330

• 论著 • 上一篇    下一篇

寻常型银屑病患者外周血髓系树突状细胞CD47的表达

钱青1,高春岩1,申宇鸿1,宋世珅1,郭乃洲2,高雯2   

  1. 1北京市和平里医院，北京，100013；2盐城市第一人民医院，盐城，224000
  • 出版日期:2020-06-15 发布日期:2020-06-04
  • 通讯作者: 高雯，E-mail: 344235345@qq.com

Expression of CD47 in peripheral blood myeloid dendritic cells in the patients with psoriasis vulgaris

QIAN Qing1, GAO Chunyan1, SHEN Yuhong1, SONG Shishen1, GUO Naizhou2, GAO Wen2   

  1. 1 Peking Hepingli Hospital, Beijing 100013, China; 2 Yancheng People's First Hospital, Yancheng 224000, China
  • Online:2020-06-15 Published:2020-06-04
  • Contact: GAO Wen, E-mail: 344235345@qq.com

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摘要： 目的：检测寻常型银屑病患者外周血髓系树突状细胞（mDCs）表面整合素相关蛋白（CD47）的表达水平。方法：流式细胞仪检测寻常型银屑病患者和健康对照者外周血mDCs表面CD47及CD4+调节性T细胞（Treg）表达水平；Luminex液相芯片技术检测上清液IL-23、IL-17和TNF-α浓度。结果：共检测46例患者和46名健康对照，患者组mDCs细胞CD47表达水平为32.8低于对照组的63.6，差异有统计学意义（P<0.05）。患者组mDCs细胞CD47表达水平与银屑病皮损面积和严重程度指数（PASI）负相关（r=-0.651, P<0.05）；患者组Treg细胞比例显著低于对照组，差异具有统计学意义（P<0.05）；IL-23、IL-17和TNF-α浓度显著高于对照组，差异具有统计学意义（P<0.05）。结论：银屑病外周血mDCs表面CD47及Treg细胞表达下降。

关键词: 寻常型银屑病, 髓系树突状细胞, 整合素相关蛋白, 调节性T细胞

Abstract: Objective: To detect the level of cluster of differentiation 47 (CD47) on myeloid dendritic cells (mDCs) in peripheral blood of the patients with psoriasis vulgaris. Methods: The level of CD47 and Treg cells in the patients with psoriasis vulgaris and healthy controls was detected by flow cytometry. The levels of IL-23, IL-17 and TNF-α in the supernatant were measured by Luminex liquid chip technology. 
Results: Forty-six patients and 46 controls were collected. The level of CD47 on mDCs in the patients was 32.8, which was lower than that in the controls (63.6±12.5), with a significant difference (P<0.05). There was a negative correlation between the level of CD47and psoriasis severity index (PASI) (r=-0.651, P<0.05). The percentage of CD4+ regulatory T cells was significantly lower in the patients than in the controls, with a significant difference (P<0.05). The levels of IL-23, IL-17 and TNF-α were significantly higher in the patients than in the controls (Ps<0.05). Conclusion: The levels of CD47 and Treg cells in the patients with psoriasis vulgaris are decreased.

Key words: psoriasis vulgaris, myeloid dendritic cells, CD47, regulatory T cells