China Journal of Leprosy and Skin Diseases ›› 2020, Vol. 36 ›› Issue (1): 3-7.doi: 10.12144/zgmfskin202001003

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Rule of Propionibacteria acnes biofilm model in vitro

LIU Yuzhen1, ZENG Rong1, XU Haoxiang1, DUAN Zhimin1, LIN Tong2, LI Min1   

  1. 1 Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing 210042, China;  
    2 Jiangsu Key Laboratory of Molecular Biology for Skin Diseases ＆ STDs，Nanjing 210042，China
  • Online:2020-01-15 Published:2020-03-16
  • Contact: LI Min, E-mail：drlimin@sina.cn；LIN Tong，E-mail：ddlin@hotmail.com

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Abstract: Objective: To construct the Propionibacteria acnes (P. acnes) biofilm model and to study the regularity of the construction of P. acnes biofilm model. Methods: The P. acnes biofilm was constructed in vitro. The structure, thickness and vitality were dynamically detected at 0, 1, 2, 3, 4, 5, 6, 7, 8, 10, 15 and 20 day by laser confocal microscopy (CLSM) and XTT reduction assay. Results: P. acnes experienced the process of biofilm formation, dissemination and re-formation within 20 days. The thickness and vitality  reached up to their peaks (29.8±2.167)μm and 0.620±0.063 at  8th day. Conclusion: P. acnes biofilm cultured in vitro for 8 days reached the best mature status.

Key words: Propionibacteria acnes, biofilm, CLSM