China Journal of Leprosy and Skin Diseases ›› 2021, Vol. 37 ›› Issue (6): 337-342.doi: 10.12144/zgmfskin202106337

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Antifungal sensitivity and relevant molecular mechanisms of two atypical Aspergillus fumigatus

JIANG Yuan1, ZHANG Caiyun2, KONG Qingtao3, SANG Hong3   

  1. 1 Department of Dermatology, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China; 2 Department of Dermatology, Nanjing Hospital of Chinese Medicine, Nanjing 210001, China; 3 Department of Dermatology, Eastern Theater General Hospital, Nanjing 210002, China
  • Online:2021-06-15 Published:2021-05-27
  • Contact: SANG Hong, E-mail: sanghong@nju.edu.cn

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Abstract: Objective: To compare the difference of in antifungal drug sensitivity and morphological related molecular mechanisms between the control strain Af293 and the two newly  clinical strain of atypical Aspergillus fumigatus (A1j and A2j). Methods: The antifungal susceptibility of A2j, A1j and Af293 to amphotericin B (AMB), itraconazole (ITC) and voriconazole (VRC) was detected by modified agar dilution susceptibility test. The method of was used to study the genes related to sporulation (brlA and wetA) were detected in A1j and A2j by RT-PCR and PCR. Results: Amphotericin B (AMB), itraconazole (ITC) and voriconazole (VRC) could inhibite the bacterial colonies of A1j, A2j and Af293, with no significant difference. After spores were induced for 6 hours, the expression level of brlA in Af293 strain was increased, but that of brlA and wetA in A1j and A2j strain was no change. The sequencing found a mutation of 597_600delTCCT of brlA in A2j and no mutation was found in A1j. Conclusion: There was no significant difference of the antifungal susceptibility of A2j, A1j and Af293 to antifungal drug. There was a mutation in sporulation gene brlA related to morphological abnormality of strain A2j. 

Key words: Aspergillus fumigatus, spores, melanin, drug sensitivity test, sporulation genes