Abstract: Objective: To study the correlation between HIV-1 infection and gene polymorphisms of CCR5, CCR2, CXCR4 and SDF1 as HIV coreceptors in population of men who have sex with men (MSM) of Han nationality in Shandong Province. Methods:HIV-1 antibody positive cases from MSM were collected in the case group, and HIV antibody negative cases were collected in the control group. The mutations of allelic polymorphisms of △32 (rs333) and 59029A/G(rs1799987) of C-C chemokine receptor 5 (CCR5), as well as of the gene exon regions of CCR2, SDF1 and CXCR4 were detected by polymerase chain reaction (PCR) and DNA sequencing. The differences in genotype distribution between the case group and the control group were compared, and  correlation of the differences with HIV-1 infection was analyzed. Results: There were 102 patients and 91 cases in the case group and control group. Heterozygous and homozygous mutations were not detected at rs333. The genotype difference between the case group and the control group of rs1799987A/G locus variation could be the risk factor of the HIV-1 infection ［OR=2.998, 95% CI: 1.034-8.696］. The mutation frequencies of rs1799987A/G, rs1799864, rs1799865 and CXCR4, SDF1 are 59.59%, 19.95%, 26.61%, 11.92% and 0.26%, respectively. There was a significant difference of polymorphism analysis of sequencing products of the third exon of CCR2 between the case group and the control group, which had no correlation with the HIV-1 infection. Conclusion:There is no △32 mutation of CCR5 gene and protective effect on HIV-1 infection among MSM. The allelic mutation frequency of 59029A/G is high (59.59%) . There was no correlation between HIV-1 infection and the gene polymorphisms of CCR5, CCR2, SDF1 and CXCR4.

Key words: MSM, chemokine receptor, AIDS, gene polymorphism