China Journal of Leprosy and Skin Diseases ›› 2022, Vol. 38 ›› Issue (6): 349-354.doi: 10.12144/zgmfskin202206349

• Original Articles • Previous Articles     Next Articles

Effect of clascoterone on the proliferation, lipid synthesis and inflammatory factors expression of human SZ95 sebocytes in vitro

YE Feng1, MO Xiaohui1, HU Tingting1, LI Xin1, WEI Ziyu1, LI Jiaqi1, CHEN Guangjie2, Christos C. Zouboulis3, JU Qiang1   

  1. 1 Department of Dermatology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200127, China; 
    2 Department of Immunology and Microbiology, School of Medicine, Shanghai Jiaotong University, Shanghai, 200025, China; 
    3 Departments of Dermatology, Venereology, Allergology and Immunology, Dessau Medical Center, Brandenburg Medical School Theodore Fontane, Dessau 06847, Germany
  • Online:2022-06-15 Published:2022-04-14
  • Contact: JU Qiang, E-mail: qiangju@aliyun.com

PDF (PC)

355

Like

Abstract: Objective: To determine the effect of clascoterone on androgen-induced human sebocytes proliferation, lipid synthesis and inflammatory factors expression in vitro and explore its anti-acne mechanism. Methods: The immortalized human sebocytes SZ95 were cultrured in vitro and then divided into Dihydrotestosterone (DHT) group, clascoterone group and clascoterone+DHT group. The cell proliferation, intracellular neutral lipids, lipid synthesis-related proteins expression, inflammatory factors and transcription level of cytokines was detected by CCK8, nile red staining and western blot, enzyme-linked immunosorbent assay and RT-qPCR respectively. Results: Compared with the DHT group, the number of cells in the clascoterone+DHT group significantly decreased (P<0.01) after 24-hour treatment. After treated with linoleic acid (LA), the level of neutral lipids in the clascoterone+DHT group was significantly  lower than that in the DHT group (P<0.0001). The lipid synthesis-related proteins such as PPARγ, PLIN2, FASN and SCD1, the transcription levels of IL-1α, IL-1β, IL-6, IL-8 and the corresponding protein levels in the clascoterone+DHT group was significantly  lower than those in the DHT group (Ps<0.05) . Conclusion: Clascoterone has a partly androgen-independent regulatory effect on human sebocytes proliferation, lipid synthesis-related genes and inflammatory factors in vitro, which might be one of anti-acne mechanisms.

Key words: clascoterone, sebocytes, acne vulgaris