Abstract: Pseudomonas putida can cause a variety of fish septic infection, but which effect on humans has not been paid much attention. Pseudomonas putida was separated from the wound on the left lower limb of a 63-year-old male, which confirmed by the biochemical identification and 16s RNA sequencing. The results of drug sensitivity test showed that the isolate was resistant to ampicillin, multiple cephalosporin antibiotics, carbapenem antibiotics, and cotrimoxazole. Further examination of resistance genes revealed that the isolate carried the carbapenem-resistance gene blaVIM-2. According to the results of drug sensitivity test, the patient was treated with ceftazidime injection 2 g, twice a day for 14 days, and fusidic acid cream was applied additionally, twice a day, and the lesion healed completely after 2 months.

Key words: Pseudomonas putida, histopathological examination, bacterial isolation, drug sensitivity test, resistance genes