中国麻风皮肤病杂志 ›› 2020, Vol. 36 ›› Issue (3): 136-141.doi: 10.12144/zgmfskin202003136

• 论著 • 上一篇    下一篇

病原相关分子模式对人3D皮肤皮脂腺模型表皮细胞增殖与分化影响的研究

李昕1,曹珂1,魏子妤1,侯霄枭1,胡婷婷2,潘展砚1,吴琼1,Christos C. Zouboulis3,鞠强1   

  1. 1上海交通大学医学院附属仁济医院皮肤科，上海，200127；2上海交通大学医学院附属仁济医院南院皮肤科，上海，201112；3 Departments of Dermatology, Venereology, Allergology and Immunology, Dessau Medical Center, Brandenburg Medical School Theodor Fontane, Germany
  • 出版日期:2020-03-15 发布日期:2020-03-30
  • 通讯作者: 鞠强，E-mail: qiangju@aliyun.com

Effects of pathogen-associated molecular patterns on proliferation and differentiation of the epidermal cells in 3D-SeboSkin model

LI Xin1, CAO Ke1,  WEI Ziyu1, HOU Xiaoxiao1, HU Tingting2, PAN Zhanyan1, WU Qiong1, Christos C. Zouboulis3, JU Qiang1#br#   

  1. 1 Department of Dermatology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200127, China; 2 Department of Dermatology, South Campus, Ren Ji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 201112, China; 3 Departments of Dermatology, Venereology, Allergology and Immunology, Dessau Medical Center, Brandenburg Medical School Theodor Fontane, Germany
  • Online:2020-03-15 Published:2020-03-30
  • Contact: JU Qiang, E-mail: qiangju@aliyun.com

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摘要： 目的：明确病原相关分子模式(PAMPs)对人表皮细胞增殖与分化的影响。方法：在人皮肤和永生化SZ95皮脂腺细胞体外共培养的3D皮肤皮脂腺培养模型中加入不同浓度(2、20、200 μg/mL)的PAMPs，包括脂多糖（LPS），磷壁酸（LTA）和肽聚糖（PGN），培养7天后，使用PhotoShop软件计算表皮面积；免疫组化观察Ki67，cytokeratin 10（CK10）的表达，采用ImageJ软件计算染色面积，Image-Pro Plus软件计算每张图片的累积光密度（IOD）。 结果：在不同浓度的PAMPs(LPS， LTA， PGN)作用下，表皮厚度总体较对照组呈现剂量依赖性增加；此外，表皮基底层及角质层细胞Ki67及CK10的表达也呈现不同程度的增加。结论：PAMPs具有体外促进表皮细胞的增殖与分化的作用，皮肤正常微生物可能在维护皮肤屏障功能上具有重要的生物学作用。

关键词: 3D皮肤皮脂腺模型, 病原相关分子模式, 表皮, 增殖, 分化

Abstract: Objective: To determine the effects of pathogen-associated molecular patterns (PAMPs) on proliferation and differentiation of epidermal cells. Methods: The proliferation and differentiation of epidermal cells invivo 3D-SeboSkin model were detected after treated with PAMPs, namely lipopolysaccharide (LPS), lipoteichoic acid (LTA) and peptidoglycan (PGN) in different concentrations (2,20,200 μg/mL). After culture for 7 days, the epidermal skin area was calculated using PhotoShop software. The proliferation and differentiation of epidermal cells were detected by immunohistochemical labeling with Ki67 and cytokeratin 10 (CK10). The stained area and the integrated optical density (IOD) of each image were calculated by the Image J software and the Image-Pro Plus software respectively. Results: After the stimulation with LPS, LTA and PGN, the epidermal thickness was increased in a concentration-dependent manner. In addition, the expression of Ki67 in the basal epidermal layer and of CK10 at the suprabasal epidermis was enhanced. Conclusion: PAMPs promote the proliferation and differentiation of human epidermal keratinocytes in vitro, indicating that skin microbes may play an important role in mantaining a normal epidermal biology and skin barrier function.

Key words: 3D-SeboSkin model, pathogen-associated molecular patterns, epidermal, proliferation, differentiation