中国麻风皮肤病杂志 ›› 2022, Vol. 38 ›› Issue (8): 524-529.doi: 10.12144/zgmfskin202208524

• 论著 • 上一篇    下一篇

过表达EphB6与BRAFi在抑制黑素瘤转移中的协同作用

刘娜1,2,李品3,王齐霞4,李明明5,黄淑红4,党宁宁5   

  1. 1潍坊医学院，潍坊，261000；2山东第一医科大学附属济南市中心医院，济南，251003；3山东第一医科大学（山东省医学科学院）医学科技创新中心，济南，250117；4山东第一医科大学（山东省医学科学院）基础医学院，济南，250117；5山东第一医科大学附属省立医院，济南，250021
  • 出版日期:2022-08-15 发布日期:2022-06-22

Synergistic effect of EphB6 overexpression and BRAFi treatment in inhibiting the metastatic progression of melanoma

LIU Na1,2, LI Pin3, WANG Qixia4, LI Mingming5, HUANG Shuhong4, DANG Ningning5   

  1. 1 Weifang Medical University, Weifang 261000, China; 2 Central Hospital Affiliated to Shandong First Medical University, Jinan 251003, China; 3 Medical Science and Technology Innovation Center of Shandong First Medical University (Shandong Academy of Medical Sciences), Jinan 250117, China; 4 Preclinical Medicine School of Shandong First Medical University (Shandong Academy of Medical Sciences), Jinan 250117, China; 5 Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, China
  • Online:2022-08-15 Published:2022-06-22

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摘要： 目的：本研究旨在探讨EphB6过表达和BRAF抑制剂(BRAFi)治疗对抑制黑素瘤转移进展过程中的协同作用。方法：从Pubchem数据库中下载维莫非尼和达帕非尼靶向基因，通过GSE141484和GSE22838芯片中分析疾病表达具有差异的基因，鉴定出EphB6。通过CCK-8、克隆形成、伤口愈合和transwell实验检测过表达EphB6并且BRAFi处理分别对A375和1205Lu细胞活力和运动能力的独立效应和协同效应。结果：过表达EphB6可显著抑制A375和1205Lu细胞的增殖、克隆形成，明显阻碍细胞的增殖速度，促进维莫非尼和达帕非尼对A375和1205Lu细胞活力和运动能力的抑制作用。结论：过表达EphB6可显著抑制黑素瘤的转移进展，有利于黑素瘤的BRAFi治疗。

关键词: 黑素瘤, BRAF抑制剂, EphB6, 增殖, 侵袭

Abstract: Objective: To indicate the synergistic effect of EphB6 overexpression and BRAF inhibitor (BRAFi) treatment in inhibiting the metastatic progression of melanoma. Methods: The vemurafenib and dabrafenib targeted genes were downloaded from the Pubchem database, while the disease differentially expressed genes were analyzed from GSE141484 and GSE22838 chips, and the EphB6 was identified. The independent and synergistic effect EphB6 overexpression with BRAFi treatment on the viability and motility of A375 and 1205Lu cells were detected by using CCK-8, clone formation, wound-healing and transwell assays. Results: EphB6 overexpression significantly inhibited the proliferation, clone formation of A375 and 1205Lu cells, and potiently hindered the speed of cells through matrigel and wound healing. Furthermore, EphB6 overexpression promoted the suppressor effect of vemurafenib and dabrafenib on A375 and 1205Lu cells viability and motility. Conclusion: EphB6 overexpression significantly inhibited the metastatic progression of melanoma, and was beneficial to the BRAFi therapy of melanoma.

Key words: melanoma, BRAF inhibitor, EphB6, proliferation, invasion