中国麻风皮肤病杂志 ›› 2021, Vol. 37 ›› Issue (11): 696-699.doi: 10.12144/zgmfskin202111696

• 论著 • 上一篇    下一篇

葡萄籽原花青素对人皮肤鳞状细胞癌A431细胞增殖和凋亡的影响

李晓民1,梁韦巍1,韩冬1,刘爱芹2,韩志强1   

  1. 1山东省潍坊市皮肤病防治所，潍坊，261061；
    2山东省潍坊市安丘市妇幼保健医院，潍坊，262100
  • 出版日期:2021-11-15 发布日期:2021-09-13
  • 通讯作者: 李晓民，E-mail: 309771921@qq.com

Effect of grape seed proanthocyanidin on proliferation and apoptosis of human skin squamous cell carcinoma A431 cells

LI Xiaomin1, LIANG Weiwei1, HAN Dong1, LIU Aiqin2, HAN Zhiqiang1   

  1. 1 Weifang Institute of Dermatology, Weifang 261061, China; 2 Anqiu Women and Children's Hospital of Weifang City in Shandong Province, Weifang 262100, China
  • Online:2021-11-15 Published:2021-09-13
  • Contact: LI Xiaomin, E-mail: 309771921@qq.com

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摘要： 目的：明确葡萄籽原花青素（GSP）对人皮肤鳞状细胞癌A431细胞增殖和凋亡的影响。方法：体外培养A431细胞，用不同浓度的GSP（5、10、20、40、80 μg/mL）处理，采用MTT实验和克隆形成实验检测细胞活力和增殖能力，Western blot检测细胞通路PI3K/Akt/GSK-3β信号转导通路蛋白的表达。结果：随着GSP浓度增加，A431细胞活力及细胞克隆逐渐下降，细胞凋亡逐渐增加。GSP处理后A431细胞中p-PI3K、p-Akt和p-GSK-3β表达水平明显降低。结论：GSP可以诱导A431细胞凋亡和抑制A431细胞增殖，其机制可能与抑制PI3K/Akt/GSK-3β信号通路活化有关。

关键词: 葡萄籽原花青素, 人皮肤鳞状细胞癌细胞, 增殖, 凋亡

Abstract: Objective: To determine the effect of grape seed proanthocyanidins (GSP) on proliferation and apoptosis of human skin squamous cell carcinoma A431 cells. Methods: A431 cells were cultured in vitro and treated with different concentrations of GSP (5, 10, 20, 40, 80 μg/mL). The cell viability and proliferation were detected by MTT assay and colony formation assay respectively. The protein level of PI3K/Akt/GSK-3β signal transduction pathway was detected by Western blot. Results: As the increasing of GSP concentration, the cell viability and cell clone level of A431 decreased gradually, apoptosis rate increased. The protein level of p-PI3K, p-Akt, p-GSK-3β decreased after treated with GSP. Conclusion: GSP can induced apoptosis and inhabited proliferation of A431 cells, which may be related with the activation of PI3K/Akt/GSK-3β signaling pathway.

Key words: grape seed proanthocyanidins, human skin squamous cell carcinoma, proliferation, apoptosis