中国麻风皮肤病杂志 ›› 2019, Vol. 35 ›› Issue (8): 476-479.doi: 10.12144/zgmfskin201908476

• 论著 • 上一篇    下一篇

萝卜硫素通过下调Cox-2/Akt/GSK3β信号传导抑制人皮肤鳞状细胞癌A431细胞增殖

杨丽亚  刘彩玉   

  1. 恩施土家族苗族自治州中心医院皮肤科,湖北恩施,445000
  • 出版日期:2019-08-15 发布日期:2019-08-19
  • 通讯作者: 刘彩玉，E-mail：3438810851@qq.com

Sulforaphane inhibits the proliferation of skin squamous cell carcinoma A431 cells through down-regulating Cox-2/Akt/GSK3β cascade

YANG Liya, LIU Caiyu   

  1. Department of Dermatology, Central Hospital of En-shi Autonomous Prefecture, Enshi 445000，China
  • Online:2019-08-15 Published:2019-08-19
  • Contact: LIU Caiyu, E-mail：3438810851@qq.com

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摘要： 目的：明确萝卜硫素对人皮肤鳞状细胞癌A431细胞株增殖、凋亡的影响及其对Cox-2/Akt/GSK3β信号传导相关蛋白水平的影响。方法：体外培养皮肤鳞状细胞癌A431细胞株，分为不同剂量（30 μM，60 μM，120 μM）萝卜硫素组、单纯顺铂组及顺铂+萝卜硫素组，采用CCK8法、流式细胞术分别检测A431细胞相对存活率及凋亡率，Western Blot检测了A431细胞中Survivin、Casepase-3、Cox-2、Akt、p-Akt、GSK3β及p-GSK3β蛋白的表达水平。结果：与对照组比较，60 μM及120 μM萝卜硫素组、单纯顺铂组及顺铂+萝卜硫素组细胞相对存活率显著降低（均P<0.05），细胞凋亡率显著增高（均P<0.05），Survivin、Cox-2、p-Akt、p-GSK3β蛋白表达水平显著降低（均P<0.05），而Caspase-3蛋白表达水平显著升高(P<0.05)；120μM萝卜硫素组与顺铂组比较，细胞相对存活率、细胞凋亡率，Survivin、Cox-2、p-Akt、p-GSK3β蛋白表达水平及Caspase-3蛋白表达，无明显统计学差异(P>0.05)。结论：萝卜硫素可通过诱导A431细胞凋亡、抑制细胞增殖发挥抗肿瘤作用，其机制可能与抑制Cox-2/Akt/GSK3β信号通路活化有关。

关键词: 萝卜硫素, 人皮肤鳞状细胞癌细胞, 增殖, 凋亡, Cox-2/Akt/GSK3β信号通路

Abstract: Objective: To determine the effects of sulforaphane on the proliferation and apoptosis of human skin squamous cell carcinoma A431 cell line and the expression level of Cox-2/Akt/GSK3β related protein. Methods: The human skin squamous cell carcinoma A431 cell line was cultured in vitro and divided into the different dose of sulforaphane groups (30 μM, 60 μM, 120 μM), cisplatin group, and sulforaphane+cisplatin group. The relative survival rate and apoptotic rate of A431 cells were detected by CCK8 assay and flow cytometric technology, respectively. The expression levels of Survivin, Casepase-3, Cox-2, Akt, p-Akt, GSK3β and p-GSK3β proteins in A431 cells were detected by Western Blot. Results: Compared with control group, the relative survival rate of A431 cells in the dose of 60 μM, 120 μM of sulforaphane groups, cisplatin group, and sulforaphane+cisplatin group were significantly decreased (P<0.05), apoptotic rate of A431 cells were greatly increased (P<0.05), the expression levels of Survivin, Cox-2, p-Akt, and p-GSK3β were greatly decreased (P<0.05), while the expression level of Caspase-3 was significantly increased (P<0.05). There was no significant difference in the relative survival rate, apoptotic rate, expression levels of Survivin, Cox-2, p-Akt, and p-GSK3β and Caspase-3 between the dose of 120 μM of sulforaphane group and cisplatin group (P>0.05). Conclusion: Sulforaphane may play an anti-tumor effect by inducing human skin squamous cell carcinoma apoptosis and suppressing their proliferation. The mechanism may be associated with the decreased activation of Cox-2/Akt/GSK3β pathway.

Key words: sulforaphane, human skin squamous cell carcinoma, proliferation, apoptosis, Cox-2/Akt/GSK3β pathway