中国麻风皮肤病杂志 ›› 2020, Vol. 36 ›› Issue (6): 327-329.doi: 10.12144/zgmfskin202006327

• 论著 • 上一篇    下一篇

多位点序列分型法对梅毒螺旋体基因分型的检测

任卓1，2,张园1，2,赵伟2,李珍2,裴晓燕1，2,杨永华1，2,田洪青1，2   

  1. 1济南大学山东省医学科学院医学与生命科学学院，济南，250000;2山东第一医科大学附属皮肤病医院（山东省皮肤病医院），山东省皮肤病性病防治研究所，济南，250022
  • 出版日期:2020-06-15 发布日期:2020-06-04
  • 通讯作者: 田洪青，E-mail: tianhq2006@126.com

Detection of Treponema Pallidumgene type by multilocus sequence typing

REN Zhuo1,2, ZHANG Yuan1,2, ZHAO Wei2, LI Zhen2, PEI Xiaoyan1,2, YANG Yonghua1,2, TIAN Hongqing1,2   

  1. 1 School of Medicine and Life Sciences, University of Jinan-Shandong Academy of Medical Sciences, Jinan 250000, China; 2 Shandong Provincial Hospital for Skin Diseases & Shandong Provincial Institute of Dermatology and Veneorology, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250022, China
  • Online:2020-06-15 Published:2020-06-04
  • Contact: TIAN Hongqing, E-mail: tianhq2006@126.com

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摘要： 目的：通过多位点序列分型法(MLST)检测我院梅毒螺旋体（TP）的临床样本DNA基因型。方法：收集梅毒患者皮损棉拭子，提取其中的TP DNA。提取的DNA通过荧光实时定量PCR证实为TP DNA；使用MLST法对样本进行分析。结果：本研究共检测55例DNA标本，发现9种MLST分型，1.1.8分型为42例，3.2.3分型为5例，1.1.1分型为2例，24.1.8分型为1例，25.1.8为1例，1.52.8为1例，1.53.8为1例，1.1.17为1例，25.3.1为1例。其中24.1.8，25.1.8，1.52.8，1.53.8，1.1.17，25.3.1为首次报道的分型。所有分型都存在对大环内酯类药物耐药。结论：我院患者的梅毒螺旋体基因型存在多样性，其中优势分型为1.1.8，各分型都对大环内酯类药物耐药。

关键词: 梅毒螺旋体, 多位点序列分型

Abstract:

Objective: To determine the DNA gene types of Treponema Pallidum in the patients with syphilis in our hospital through multilocus sequence typing (MLST). Methods: Samples were collected from clinical lesions with cotton swabs and TP-DNA was extracted and confirmed by quantitative real-time PCR. The genetypes of TP were detected by MLST. Results: Fifty-five samples were detected and 9 genetypes were identified, including type 1.1.8 in 42 cases, type 3.2.3 in 5, type 1.1.1 in 2, type 24.1.8 in 1, type 25.1.8 in 1, type 1.52.8 in 1, type 1.53.8 in 1, type 1.1.17 in 1, type 25.3.1 in 1. Type 24.1.8, 25.1.8, 1.52.8, 1.53.8, 1.1.17 and 25.3.1 were reported firstly. All types of TP were resistant to macrolides. Conclusion: TP genetypes are variegated in the patients with syphilis in our hospital. The dominant type is 1.1.8, and all types of TP are resistant to macrolide.

Key words: Treponema Pallidum, multilocus sequence typing