中国麻风皮肤病杂志 ›› 2019, Vol. 35 ›› Issue (5): 280-283.doi: 10.12144/zgmfskin201905280

• 论著 • 上一篇    下一篇

临床梅毒螺旋体菌株兔感染模型构建

张园1,2 #  任卓1,2 #  侯建玲2  郑荣涛2  李中伟2  刘建新2  庞静2  黄娜2  田洪青1,2#br#   

  1. 1济南大学山东省医学科学院医学与生命科学学院，济南，250000
    2山东省皮肤病性病防治研究所，山东省皮肤病医院，济南，250022
    #共同第一作者
  • 出版日期:2019-05-15 发布日期:2019-06-03
  • 通讯作者: 田洪青,E-mail: thq2006@126.com

Construction of infection model of Treponema pallidum strains clinically collected in rabbits

ZHANG Yuan1,2#, REN Zhuo1,2#, HOU Jianling2, ZHENG Rongtao2, LI Zhongwei2, LIU Jianxin2, PANG Jing2, HUANG Na2, TIAN Hongqing1,2   

  1. 1. School of Medicine and Life Sciences, University of Jinan－Shandong Academy of Medical Sciences, Jinan 250000, China; 2. Shandong Dermatology Hospital, Shandong Provincial Institute of Dermatology and Venereology, Jinan 250022, China #: equal contributiom
  • Online:2019-05-15 Published:2019-06-03
  • Contact: TIAN Hongqing, E-mail: thq2006@126.com

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摘要： 目的：构建临床梅毒螺旋体菌株梅毒兔感染模型。方法：收集梅毒患者梅毒螺旋体菌株，制备菌悬液，将悬液注入新西兰兔睾丸并连续分离传代，观察新西兰雄兔临床症状及血清学变化情况。结果：共收集临床梅毒螺旋体菌6株 (A-F)，接种成功5株—A、C、D、E、F菌株，分别于接种后第28天、7天、14天、32天、35天检测到TPPA转阳。将获得的临床菌株A继续传代，接种6天开始睾丸出现肿胀，平均7.7天TPPA转阳。结论：通过兔睾丸接种的方法，可以成功分离传代临床梅毒螺旋体菌株。

关键词: 梅毒, 梅毒螺旋体, 动物模型, 兔

Abstract: Objective: To establish the animal model of Treponema pallidum strain clinically collected. Methods: The clinically isolated Treponema pallidum strain was prepared for suspension. The suspension was injected into New Zealand rabbit and continuously passaged to next generations. The clinical symptoms of rabbit and serological changes were observed and tested. Results: A total of 6 strains of Treponema pallidum (A-F) were collected and 5 strains (A, C, D, E, F) were successfully inoculated. TPPA conversion was detected on days 28, 7, 14, 32 and 35, respectively. The strain A was passaged and the testicles showed swelling after inoculated strain A for 6 days and positive TPPA conversion after 7.7 days on average. Conclusion: The strain of Treponema pallidum can be successfully isolated clinically and passaged by rabbit testicular inoculation method.

Key words: syphilis, Treponema pallidum, animal model, rabbit