中国麻风皮肤病杂志 ›› 2021, Vol. 37 ›› Issue (1): 8-12.doi: 10.12144/zgmfskin202101008

• 论著 • 上一篇    下一篇

抑制树突细胞MMP-13基因对咪喹莫特诱导的小鼠银屑病样炎症模型的影响

韩阳,张芯蕊,陈娟,刘文琪,李晓东   

  1. 沈阳医学院附属中心医院，沈阳，110024
  • 出版日期:2021-01-15 发布日期:2021-01-04
  • 通讯作者: 李晓东，E-mail: lilicmu@163.com

The effect of inhibition of MMP-13 gene in dendritic cell on a mouse model of imiquimod-induced psoriasis

HAN Yang, ZHANG Xinrui, CHEN Juan, LIU Wenqi, LI Xiaodong   

  1. Central Hospital Affiliated To Shenyang Medical College, Shenyang 110024, China
  • Online:2021-01-15 Published:2021-01-04
  • Contact: LI Xiaodong, E-mail: lilicmu@163.com

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摘要： 目的：明确抑制树突细胞MMP-13基因对咪喹莫特诱导的小鼠银屑病样炎症模型的影响。方法：构建树突细胞MMP-13基因特异性敲除的小鼠模型（MMP-13LOX/LOX），应用咪喹莫特诱导小鼠银屑病样皮损的发生。将小鼠分为野生型对照组、MMP-13LOX/LOX对照组、咪喹莫特诱导的野生型实验组和咪喹莫特诱导的MMP-13LOX/LOX实验组，分析不同组别小鼠皮损临床表现并记录PASI评分，检测各组小鼠皮损中IL-1β、IL-6、IL-23和IL-17A表达。结果：与咪喹莫特诱导的野生型实验组小鼠比较，咪喹莫特诱导的MMP-13LOX/LOX实验组银屑病皮损表现相比更加温和，仅有轻度的棘层肥厚和真皮细胞浸润，皮损中IL-1β、IL-6表达下调，而IL-23和IL-17A表达无明显改变。结论：抑制树突细胞MMP-13基因可降低炎症因子IL-6、IL-1β的表达及减弱小鼠皮肤银屑病炎症反应。

关键词: MMP-13, 银屑病, 细胞因子

Abstract: Objective: To determine the effect of inhibition of MMP-13 gene in dendritic cell on a mouse model of imiquimod-induced psoriasis. Methods: A mouse model of MMP-13 gene specific knockout in dendritic cells was constructed, and then, the psoriasis lesion was induced by imiquimod. Mice were divided into wild-type control group, MMP-13LOX/LOX control group, wild-type imiquimod group, MMP-13LOX/LOX imiquimod group. The clinical manifestations of skin lesions in each group and the psoriasis severity index (PASI) were assessed. The expressions of IL-1, IL-6, IL-23 and IL-17A in mice lesions were detected. Results: Compared with the wild-type imiquimod group, the lesions manifestation of the mice in MMP-13LOX/LOX imiquimod group were milder, with only mild acanthosis and dermal cell infiltration. The expression level of IL-1 and IL-6 was lower and there was no significant difference in the expression level of IL-23 and IL-17A. Conclusion: Inhibition of MMP-13 gene of dendritic cells can decrease the level of IL-6 and IL-1β and inhibit the inflammation of psoriasis mouse model.

Key words: MMP-13, psoriasis, cytokines