中国麻风皮肤病杂志 ›› 2025, Vol. 41 ›› Issue (5): 337-342.doi: 10.12144/zgmfskin202505337

• 论著 • 上一篇    下一篇

低温等离子体治疗豚鼠皮肤真菌与细菌复合感染的疗效评价

吴晋彤1,2,王溯2,3,章程4,黄邦斗4,王睿2,李承新2,5   

  1. 1解放军医学院，北京，100853； 2解放军总医院第一医学中心皮肤科，北京，100853； 3南开大学医学院，天津，300071； 4中国科学院电工研究所，北京，100190； 5肾脏疾病全国重点实验室，北京，100853
  • 出版日期:2025-05-15 发布日期:2025-05-15

Efficacy of cold atmospheric plasma in a guinea pig model of fungal-bacterial co-infection

WU Jintong1,2, WANG Su2,3, ZHANG Cheng4, HUANG Bangdou4, WANG Rui2, LI Chengxin2,5   

  1. 1 Medical School of Chinese PLA, Beijing 100853, China; 2 Department of Dermatology, the First Medical Center of Chinese PLA General Hospital, Beijing 100853, China; 3 School of Medicine, Nankai University, Tianjin 300071, China; 4 Institute of Electrical Engineering, Chinese Academy of Sciences, Beijing 100190, China; 5 State Key Laboratory of Kidney Diseases, Beijing 100853, China
  • Online:2025-05-15 Published:2025-05-15

PDF (PC)

17

摘要： 目的：评价低温等离子体（cold atmospheric plasma，CAP）对豚鼠皮肤真菌感染合并细菌定植模型的疗效。方法：35只免疫抑制豚鼠，建立红色毛癣菌、金黄色葡萄球菌复合感染模型，随机分为5组，每组7只。A组（空白组）、B组（阳性对照组，以联苯苄唑乳膏、莫匹罗星软膏处理）、C组（低剂量CAP组，30%强度即3档位）、D组（中剂量CAP组，50%强度即5档位）、E组（高剂量CAP组，80%强度即8档位）。通过临床、病原学、病理学进行疗效评估。结果：除A组外，各组治疗前后皮损评分差异均具有统计学意义（P＜0.05），治疗2周后A组（11.43±0.98）分，B组（1.71±0.49）分，C组（4.86±1.07）分，D组（3.43±0.54）分，E组（1.86±1.07）分，B、C、D、E组均优于A组（P＜0.001），B、D、E组间无显著差异。除A组外，各组真菌镜检、病原学培养阴性率均高于治疗前（P＜0.05），组间无显著差异。B与D、E组病理学评分比较无显著差异。结论：CAP对该模型的疗效与莫匹罗星、联苯苄唑的联合作用相当，一定范围内随剂量的增大而增加，达到一定剂量后疗效维持稳定。

关键词: 低温等离子体, 皮肤浅部真菌病, 细菌定植, 豚鼠

Abstract: Objective: To evaluate the efficacy of cold atmospheric plasma (CAP) in a guinea pig skin model of fungal-bacterial co-infection. Methods: 35 immunocompromised guinea pigs were used to establish a co-infection model with Trichophyton rubrum and Staphylococcus aureus. They were randomly divided into 5 groups, 7 in each group. Group A (blank group), group B (positive control group, treated with bifonazole cream and mupirocin ointment), group C (low dose CAP group, 30% intensity of 3 gear), group D (medium dose CAP group, 50% intensity of 5 gear), group E (high dose CAP group, 80% intensity of 8 gear). The efficacy was assessed through clinical, microbiological, and pathological examinations. Results: The differences in skin lesion scores before and after treatment were statistically significant (P＜0.05) in 4 groups (groups B-E). After 2 weeks of treatment, group A (11.43 ± 0.98), group B (1.71 ± 0.49), group C (4.86 ± 1.07), group D (3.43 ± 0.54), and group E (1.86 ± 1.07). Groups B, C, D, and E were better than group A (P＜0.001), and there was no significant difference between group B, D and E. The negative rates of fungal microscopy and microbiological cultures in groups B-E were higher than before treatment (P＜0.05), with no significant difference among groups. There were no significant difference in pathological scores between group B and D, E. Conclusion: The efficacy of CAP in this model is comparable to the combined effects of mupirocin and bifonazole, increasing with dosage within a specific range and stabilizing after reaching a certain dosage.

Key words: cold atmospheric plasma, superficial mycoses, bacterial colonization, guinea pig