中国麻风皮肤病杂志 ›› 2025, Vol. 41 ›› Issue (8): 564-570.doi: 10.12144/zgmfskin202508564

• 论著 • 上一篇    下一篇

HIV感染者肠道炎症与钙卫蛋白表达水平的相关性研究

范荟芸1,邓忠芸2,卿勇3,穰真4,崔凡1，4   

  1. 1西南医科大学临床医学院，四川泸州，646000； 2川投西昌医院，四川西昌，615000； 3成都市肛肠专科医院肛肠皮肤科，四川成都，610015； 4四川省医学科学院，四川省人民医院皮肤病性病研究所，四川成都，610031
  • 出版日期:2025-08-15 发布日期:2025-07-31

Correlation between gut inflammation and calreticulin expression in HIV-infected individuals

FAN Huiyun1, DENG Zhongyun2, QING Yong3, RANG Zhen4, CUI Fan1,4   

  1. 1 The Affiliated Hospital of Southwest Medical University, Luzhou 646000, China; 2 Sichuan Provincial People's Hospital Medical Group Chuantou Xichang Hospital, Xichang 615000, China; 3 Department of Proctology and Dermatology, Chengdu Anorectal Hospital, Chengdu 610015, China; 4 Sichuan Academy of Medical Sciences, Sichuan Provinicial People's Hospital Institute of Dermatology and Venereology, Chengdu 610031, China
  • Online:2025-08-15 Published:2025-07-31

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摘要： 目的：探讨HIV感染者肠道炎症与钙卫蛋白（S100A8/A9）的相关性。方法：选取15 例 AIDS 期患者（A组）、15 例无症状 HIV感染者（B组）、10 例健康者（C组），采集血液、粪便、肠组织。ELISA测钙卫蛋白水平，HE 染色观察肠黏膜炎症程度，免疫组化和蛋白印迹评估肠道组织中S100A8/A9表达。结果：A、B 组血清及粪便钙卫蛋白水平高于 C 组（P＜0.05），A组血清钙卫蛋白水平高于 B 组（P=0.016）。HE染色示HIV感染者肠黏膜病变加重，中性粒细胞增多（P＜0.05）。免疫组化和蛋白印迹示A、B 组肠组织 S100A8/A9 表达高于C组（P＜0.05），且A组高于B组。CD4+T细胞计数与血清、粪便及肠组织中 S100A8/A9 表达呈负相关（均P＜0.001）。结论：HIV 感染者钙卫蛋白水平与肠道炎症相关，并随病程进展而升高。

关键词: HIV感染, 肠道炎症, 钙卫蛋白（S100A8/A9）, 生物标志物, CD4+T 细胞

Abstract: Objective: To investigate the correlation between intestinal inflammation and the expression levels of calprotectin (S100A8/A9) in HIV-infected individuals. Methods: Fifteen AIDS patients (Group A), 15 asymptomatic HIV-infected individuals (Group B), and 10 healthy individuals (Group C) were selected, and blood, feces and intestinal tissues of them were collected. The calprotectin level was measured by ELISA, the degree of intestinal mucosal inflammation was observed through HE staining, the expression of S100A8/A9 in intestinal tissue was evaluated by immunohistochemistry and Western blotting. Results: The levels of serum and fecal calprotectin in Groups A and B were higher than those in Group C (P＜0.05), and the level of serum calprotectin in Group A was higher than that in Group B (P=0.016). HE staining showed that intestinal mucosal lesions in HIV-infected individuals were aggravated and neutrophils increased (P＜0.05). Immunohistochemistry and Western blotting showed that the expression of S100A8/A9 in intestinal tissues of Groups A and B was higher than that of Group C (P＜0.05), and the expression in Group A was higher than that in Group B. The count of CD4+T cells was negatively correlated with the expression of S100A8/A9 in serum, feces and intestinal tissues (Ps＜0.001). Conclusion: The calprotectin level in HIV-infected individuals is associated with intestinal inflammation and increases with the progression of the disease course.

Key words: HIV infection, gut inflammation, calprotectin (S100A8/A9), biomarker, CD4+ T cells