中国麻风皮肤病杂志 ›› 2016, Vol. 32 ›› Issue (3): 150-152.

• 论著 • 上一篇    下一篇

寻常型银屑病患者外周血中微粒子的表达

李 林，聂建军，张力文，王文菊，张大维，刘 艳，陈 涛   

  1. 成都市第二人民医院皮肤科 610017
  • 出版日期:2016-03-15 发布日期:2018-12-19

Expression of microparticles in peripheral blood of patients with psoriasis vulgaris

LI Lin, NIE Jianjun, ZHANG Liwen, WANG Wenju, ZHANG Dawei, LIU Yan, CHEN Tao   

  1. Department of Dermatology, Chengdu Second People’s Hospital, Chengdu 610017, China
  • Online:2016-03-15 Published:2018-12-19

PDF (PC)

92

摘要： 目的：检测微粒子在寻常型银屑病外周血中的表达。方法：高速离心法分离33例寻常型银屑病患者和33名正常人对照外周血T细胞、B细胞及单核细胞来源的微粒子，分别进行细胞特异性单克隆抗体标记，采用流式细胞仪检测三种细胞来源的微粒子数量。结果：银屑病组患者血浆中T淋巴细胞（CD3+）来源的微粒子、单核细胞（CD14+）来源的微粒子和B淋巴细胞（CD19+）来源的微粒子数量分别为（6.6±0.2）×105,（3.9±0.3）×105和（4.1±0.2）×105高于正常对照组的（2.0±0.3）×105，（1.6±0.3）×105和（1.1±0.2）×105.治疗后银屑病组患者微粒子的总数量较治疗前明显减少（P<0.01）。此外，银屑病患者血浆中微粒子水平与PASI评分具有相关性,尤其是T淋巴细胞源性微粒子。结论：微粒子的数量在寻常型银屑病外周血中明显升高，可能在银屑病的发病中起到重要作用。

关键词: 寻常型银屑病, 微粒子, PASI评分

Abstract: Objective: To detect the level of microparticles in peripheral blood of patients with psoriasis. Methods: The microparticles from T-cells, B-cells and monocyte of 33 patients with psoriasis vulgaris and 33 healthy controls were isolated by high-speed differential centrifugation. Microparticles from T-cells, B-cells and monocyte were treated with specific labeled monoclonal antibodies respectively and the number of microparticles was detected by flow cytometry. Results: The total number of microparticles derived from T cells, B cells and monocytes in patients was (6.6±0.2)×105, (3.9±0.3)×105 and (4.1±0.2)×105 and those in healthy controls was (2.0±0.3)×105，(1.6±0.3)×105 and (1.1±0.2)×105, with significant difference (P<0.05). The number of microparticles in patients decreased after treatment. And there was a positive correlation between the level of microparticles and PASI in patients with psoriasis vulgaris, especial microparticles from T-cells. Conclusion: The number of microparticles in patients with psoriasis vulgaris increased significantly, which may play a role in the pathogenesis of psoriasis vulgaris.

Key words: psoriasis vulgaris, microparticles, PASI