中国麻风皮肤病杂志 ›› 2016, Vol. 32 ›› Issue (5): 263-266.

• 论著 • 上一篇    下一篇

斑秃皮损凋亡因子Annexin V表达及与T淋巴细胞浸润的相关性

赵 莹1 张小婷2 蔡泽明3 叶艳婷4 张 斌4 章星琪4   

  1. 1 菏泽市立医院皮肤科 ，270000 2 温州医科大学附属第二医院皮肤科 ,325000 3 南昌大学第三附属医院皮肤科，330008 4 中山大学附属第一医院 皮肤科，510000
  • 出版日期:2016-05-15 发布日期:2018-12-24
  • 通讯作者: 章星琪，E-mail: xingqi.zhang@aliyun.com

Correlation between the apoptotic factor Annexin V and T lymphocytes infiltration in alopecia areata

Zhao Ying1, Zhang Xiaoting2, Cai Zeming3, Ye Yanting4, Zhang Bin4, Zhang Xingqi4.   

  1. 1.Department of Dermatology, Heze Municipal Hospital, Heze 270000，China; 2. Department of Dermatology, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China; 3. Department of Dermatology, the Third Affiliated Hospital of Nanchang University, Nanchang 330008, China; 4. Department of Dermatology, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510000, China
  • Online:2016-05-15 Published:2018-12-24
  • Contact: Zhang Xingqi, Email: xingqi.zhang@aliyun.com

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摘要： 目的： 检测斑秃皮损凋亡因子Annexin V的表达并确定其与T淋巴细胞浸润的相关性。方法：对22例斑秃患者皮损（11例早期斑秃和11例中晚期斑秃）和10例正常头皮进行CD3/CD4/CD8和Annexin V 的免疫组化双染。结果: 早期斑秃Annexin V在浅层血管和毛囊周围的阳性细胞数显著高于中晚期斑秃，而在深层毛囊周围显著低于中晚期斑秃（P<0.05）。早期斑秃深层毛囊周围T淋巴细胞浸润与Annexin V阳性细胞数量呈显著正相关，而浅层毛囊周围两者无明显相关性。结论: 早期斑秃浅层永久性毛囊细胞凋亡与T淋巴细胞浸润无相关性,而深层周期性毛囊周围T细胞浸润是毛囊细胞凋亡的主要原因。

关键词: 斑秃, 凋亡因子, T淋巴细胞

Abstract: Objective: To detect the expression of apoptotic factors Annexin V and to determine the relationship between the expression of apoptotic factors Annexin V and the T lymphocyte infiltration in alopecia areata (AA). Methods: Immunohistochemical double-staining of CD3/CD4/CD8 and Annexin V was used to detect the expression of apoptotic factors Annexin V in the lesions of 22 patients with AA (11 patients at early stage and 11 at advanced stage) and normal scalp of 10 controls. Results: The number of Annexin V-positive cells around vessels and follicular in the superficial layer of the lesions in early stage was significantly higher than that in advanced stage. However, the number in deep layer around follicular of the lesions was significantly lower than that in advanced stage (P <0.05). There was a positive correlation between the number of T lymphocyte and Annexin V positive cells in deep layer and there was no significant correlation in superficial layer. Conclusion: The apoptosis of permanent hair follicle cells in superficial layer was uncorrelated with T lymphocyte infiltration and the T cell infiltration was the main cause of the apoptosis of cyclical hair follicle cells in deep layer.

Key words: alopecia areata, apoptotic factors, T lymphocyte