中国麻风皮肤病杂志 ›› 2016, Vol. 32 ›› Issue (8): 449-152.

• 论著 •    下一篇

CXCL12对人黑素瘤细胞株M14生物学行为的影响

杨莹，倪娜娜，王光平，温斯健，宋昊，崔盘根，孙建方   

  1. 中国医学科学院北京协和医学院皮肤病研究所,南京，210042
  • 出版日期:2016-08-15 发布日期:2019-03-01
  • 通讯作者: 孙建方，E-mail: fangmin5758@aliyun.com

Effects of CXCL12 on the biological behavior of human melanoma M14 cell line

YANG Ying, NI Nana, WANG Guangping, WEN Sijian, SONG Hao, CUI Pangen, SUN Jianfang   

  1. Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing 210042, China
  • Online:2016-08-15 Published:2019-03-01
  • Contact: SUN Jianfang, E-mail: fangmin5758@aliyun.com

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摘要： 目的：明确CXCL12对人黑素瘤细胞株M14体外增殖、迁移、侵袭的影响。方法：用不同浓度的CXCL12（0、10、50、100、200 ng/mL）刺激或诱导人黑素瘤细胞株M14，CCK8法检测细胞增殖，Transwell迁移和侵袭试验检测细胞迁移和侵袭能力。用100 ng/mL的CXCL12处理人黑素瘤细胞株M14，Western blot法检测处理后不同时间点（0、5、10、20、30、60 min）的 ERK、p-ERK、AKT、p-AKT的表达。结果：CXCL12能够增强人黑素瘤细胞株M14细胞的体外增殖、迁移、侵袭能力，以及促进ERK、AKT信号通路的激活。结论：CXCL12可能与黑素瘤的进展过程相关。

关键词: 黑色素瘤, CXCL12, 肿瘤细胞生物学行为

Abstract: Objective: To investigate the effects of CXCL12 on the biological behavior of human melanoma M14 cell line. Methods: The human melanoma M14 cell line was incubated or induced by different concentrations of CXCL12 (0,10,50,100,200 ng/mL). The Cell Counting Kit-8 (CCK8), transwell migration and invasion assay were used to evaluate the proliferation, migration, and invasion of M14 cells. The Western blot was used to detect the protein expression of ERK, p-ERK, AKT, and p-AKT of M14 cells treated with 100 ng/mL CXCL12 at different times (0, 5, 10, 20, 30, 60 min). Results: CXCL12 promoted the proliferation, migration and invasion of M14 cells, and the activation of the ERK and AKT signaling pathways. Conclusion: CXCL12 may be associated with the progression of melanoma.

Key words: melanoma, CXCL12, tumor cell biological behavior