中国麻风皮肤病杂志 ›› 2018, Vol. 34 ›› Issue (10): 585-589.

• 论著 • 上一篇    下一篇

慢性自发性荨麻疹患者外周血IL-35水平检测及其对血管内皮细胞VCAM-1的抑制作用

钱青1，高春岩1，申宇鸿1，宋世珅1，于建秀2，于月红2   

  1. 1北京市和平里医院皮肤科，北京, 210000 2滨海县人民医院检验科，江苏, 224500
  • 出版日期:2018-10-15 发布日期:2018-12-04
  • 通讯作者: 于月红，E-mail：qianqingab@126.com

Detection of peripheral blood IL-35 in the patients with chronic spontaneous urticaria and the inhibitory effect of IL-35 on VCAM-1 in vascular endothelial cell

QIAN Qing1, GAO Chunyan1,SONG Shikun1,SHEN Yuhong1, YU Jianxiu2, YU Yuehong2   

  1. 1 Department of Dermatology, Peking Hepingli Hospital, Beijing 100013, China; 2 Departments of Laboratory Medicine, The People’s Hospital of Binhai County, Jiangsu 224500, China
  • Online:2018-10-15 Published:2018-12-04
  • Contact: YU Yuehong, E-mail: qianqingab@126.com

PDF (PC)

177

摘要： 目的：检测慢性自发性荨麻疹（CSU）患者外周血IL-35水平，明确其对肥大细胞活化的血管内皮细胞表面血管细胞粘附分子-1 (VCAM-1)表达的抑制效应。方法：收集30例CSU患者和30例健康对照者外周血，利用Luminex液相芯片检测血清IL-35和可溶性VCAM-1（sVCAM-1）浓度。体外培养肥大细胞，与患者外周血清共孵育24h，取上清液 、IL-35 与人脐静脉内皮细胞（HUVECs）共培养，分为空白对照组（仅含RPMI 1640培养液）、健康人上清液组（HC）、CSU上清液组（CSU）、HC+IL-35组、CSU+IL-35组，流式细胞术检测VCAM-1蛋白表达，荧光显微镜观察单个核细胞和内皮细胞的粘附。结果：与健康对照者相比，CSU患者组外周血IL-35浓度显著降低，血清sVCAM-1浓度显著升高，差异均有统计学意义（均P<0.05）。 CSU血清IL-35与sVCAM-1浓度呈负相关（r=-0.5232 P=0.003）。IL-35+CSU组内皮细胞VCAM-1表达及单个核细胞与内皮细胞粘附情况显著低于CSU组，差异均有统计学意义（均P<0.05）。结论：CSU患者外周血IL-35降低，升高IL-35浓度可抑制CSU患者肥大细胞对血管内皮细胞的活化作用。

关键词: 慢性自发性荨麻疹, 白细胞介素-35, 血管内皮细胞, 血管细胞粘附分子-1

Abstract: Objective: To detect the level of IL-35 in peripheral blood of the patients with chronic spontaneous urticaria (CSU) and to determine the inhibitory effect of IL-35 on mast cell-activated vascular cell adhesion molecule-1 (VCAM-1) in human umbilical vein endothelial cells (HUVECs). Methods：Peripheral blood from 30 patients with CSU and 30 healthy controls (HC) was collected. The concentrations of serum IL-35 and soluble VCAM-1 (sVCAM-1) were detected by Luminex 200. Mast cells were cultured in vitro and co-incubated with peripheral serum for 24 hours, then the supernatant was collected. After that, the supernatant and IL-35 were co-cultured with HUVECs, which were divided into the control group (only contains RPMI 1640 medium), healthy people supernatant group (HC), CSU supernatant group (CSU), HC+IL-35 group and CSU+IL-35 group. The level of VCAM-1 protein was detected by flow cytometry, and the adhesion of mononuclear cells and endothelial cells was detected by fluorescence microscopy. Results: Compared with the healthy controls, the concentration of serum IL-35 was significantly reduced and the serum sVCAM-1 concentration was significantly increased in the CSU patients (Ps<0.05). The concentration of serum IL-35 was negatively correlated with sVCAM-1 in CSU patients (r=-0.5232 P=0.003). The expression of VCAM-1 in HUVECs and the adhesion of PBMCs to HUVECs in IL-35+CSU group were significantly lower than those in the CSU group (Ps<0.05). Conclusions: The concentration of serum IL-35 is decreased in CSU patients and up-regulation of IL-35 concentration can inhibit the activation of vascular endothelial cells by mast cells.

Key words: chronic spontaneous urticaria, interleukin-35, endothelial cells, vascular cell adhesion molecule-1