中国麻风皮肤病杂志 ›› 2018, Vol. 34 ›› Issue (8): 453-456.

• 论著 • 上一篇    下一篇

IL-35在特应性皮炎样小鼠模型中的表达

王倩，刘伟，薛竞，王勤，张丽霞   

  1. 四川省医学科学院·四川省人民医院皮肤性病研究所，四川 成都 610072
  • 出版日期:2018-08-15 发布日期:2018-12-11
  • 通讯作者: 张丽霞，zhanglixia0310@163.com

Expression of interleukin-35 in atopic dermatitis-like murine model

WANG Qian, LIU Wei, XUE Jing, WANG Qin, ZHANG Lixia   

  1. The Institute of Dermatology and Venereology, Sichuan Academy of Medical Sciences & Sichuan Provincial People’s Hospital, Chengdu 610072, China
  • Online:2018-08-15 Published:2018-12-11
  • Contact: ZHANG Lixia, E-mail: zhanglixia0310@163.com

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摘要： 目的：检测IL-35在特应性皮炎小鼠模型中的表达。方法：将30只BALB/c小鼠随机分为模型组及对照组，每组15只，使用丙酮：橄榄油3：1溶液作为基质配置0.5% 2,4-二硝基氟苯（DNFB）经皮致敏建立特应性皮炎样小鼠模型。造模成功后使用ELISA法检测小鼠血清中IL-35、IL-4、IFN-γ、IL-17的水平，采用RT-PCR法检测皮肤组织中IL-12p35mRNA及EBI3mRNA水平。结果：模型组小鼠背部皮肤出现明显炎症，病理表现为表皮增厚、海绵水肿、真皮浅层炎细胞浸润。模型组小鼠血清中IL-35水平明显低于对照组（P<0.05），IL-4、IL-17水平明显高于对照组（P<0.05）且IL-35与IL-17水平呈负相关（P<0.05）；模型组小鼠背部皮损组织中IL-12p35及EBI3mRNA水平均低于对照组（P<0.05）。结论：IL-35可能在特应性皮炎发病中发挥作用。

关键词: 白介素-35, 特应性皮炎, 小鼠模型

Abstract: Objective: To detect the expression of IL-35 in atopic dermatitis-like murine model. Methods: Thirty BALB/c mice were randomly divided into the model group and control group (15 in each). The atopic dermatitis-like models were established by topical application of 0.5% 2,4-dinitrofluorobenzene. After successful modeling, the serum levels of IL-35, IL-4, IFN-γ and IL-17 were detected by ELISA and the expressions of IL-12p35 and EBI3 mRNA were tested by RT-PCR. Results: In the model group, the inflammation was obvious in the skin of the back The histopathology of skin lesions showed thickness of the epidermis, spongiosis and infiltration of inflammatory cells in the superficial dermis. The serum level of IL-35 in the model group was lower than that in the control group (P <0.05). The serum levels of IL-4, IL-17 in the model group were higher than those in the control group (P <0.05). There was a negative correlation between IL-35 and IL-17 levels (P<0.05). The expressions of IL-12p35 and EBI3 mRNA were lower than those in the control group (P <0.05). Conclusion: The expression levels of IL-35 may play a role in the pathogenesis of atopic dermatitis.

Key words: Interleukin-35, atopic dermatitis, mouse model