中国麻风皮肤病杂志 ›› 2016, Vol. 32 ›› Issue (5): 257-259.

• 论著 •    下一篇

雷公藤多苷对狼疮鼠肝肾组织中11β-羟基类固醇脱氢酶的影响

张 博1，方险峰2，安永涛1   

  1. 1.广西中医药大学，广西南宁 530000 2.广西中医药大学附属瑞康医院，广西南宁 530000
  • 出版日期:2016-05-15 发布日期:2018-12-24
  • 通讯作者: 方险峰，E-mail：fxf777@qq.com?
  • 基金资助:
    基金项目：国家自然科学基金（编号：81260593）， 广西壮族自治区自然科学基金（编号：2012GXNSFAA053101）， 广西壮族自治区教育厅科研项目（编号：201203YB105）

Effect of tripterygium glycosides on the expression of 11β-hydroxysteroid dehydrogenase in liver and kidney of MRL/lpr mice

ZHANG Bo 1, FANG Xianfeng 2, AN Yongtao 1   

  1. 1.Guangxi Traditional Chinese Medicine University, Nanning 530000, China; 2.Rui Kang Affiliated Hospital of Guangxi Traditional Chinese Medicine University, Nanning 530000, China
  • Online:2016-05-15 Published:2018-12-24
  • Contact: FANG Xianfeng，E-mail: fxf777@qq.com

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摘要： 目的: 明确雷公藤多苷对MRL/lpr狼疮小鼠肝脏组织中11β-羟基类固醇脱氢酶1（11β-HSD1）和肾组织中11β-羟基类固醇脱氢酶2（11β-HSD2）表达的影响。方法: 将14只MRL/lpr狼疮鼠随机分为实验组和对照组，实验组8只给予雷公藤多苷15 mg/kg灌胃，每天1次，连续15天，其余6只作为空白对照。免疫组化、RT-PCR分别检测11β-HSD的IOD值和mRNA的表达。结果: 实验组中11β-HSD1的IOD和mRNA分别为1.03±0.05和1.55±0.09，高于对照组的0.83±0.04和1.23±0.07，（P<0.05）；实验组中11β-HSD2的IOD和mRNA为0.86±0.06和1.10±0.04低于对照组中的0.90±0.05和1.15±0.06），差异无统计学意义（P>0.05）。结论: 雷公藤多苷可诱导肝脏中11β-HSD1的表达。

关键词: 雷公藤多苷, 系统性红斑狼疮, 11β-羟基类固醇脱氢酶

Abstract: Objective: To determine the effects of tripterygium glycosides (TG) on the expression of 11β-Hydroxysteroid Dehydrogenase1（11β-HSD1) in liver and 11β-Hydroxysteroid Dehydrogenase2（11β-HSD2）in kidney of MRL/lpr mice. Methods: Fourteen MRL/lpr mice were randomly divided into two groups. Eight mice in the experimental group were given TG, 15mg/kg, once a day, for 15 days. Six mice were used as blank controls. The expression of 11β-HSD1 and 11β-HSD2 was detected by immunohistochemistry and PCR．Results: The IOD and mRNA of 11β-HSD1 in the experimental group were 1.03±0.05 and 1.55±0.09, which was higher than those in the control group (0.83±0.04 and 1.23±0.07), with a significant difference (P<0.05). The IOD and mRNA of 11β-HSD2 in the experimental group were 0.86±0.06 and 1.10±0.045, which was lower than those in the control group (0.90±0.05 and 1.15±0.06), with no significant difference (P>0.05). Conclusion: Tripterygium glycosides can induce the expression of 11β-HSD1 in liver．

Key words: tripterygium glycosides, systemetic lupus erythematosus, 11β-hydroxysteroid dehydrogenase