China Journal of Leprosy and Skin Diseases ›› 2020, Vol. 36 ›› Issue (6): 327-329.doi: 10.12144/zgmfskin202006327

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Detection of Treponema Pallidumgene type by multilocus sequence typing

REN Zhuo1,2, ZHANG Yuan1,2, ZHAO Wei2, LI Zhen2, PEI Xiaoyan1,2, YANG Yonghua1,2, TIAN Hongqing1,2   

  1. 1 School of Medicine and Life Sciences, University of Jinan-Shandong Academy of Medical Sciences, Jinan 250000, China; 2 Shandong Provincial Hospital for Skin Diseases & Shandong Provincial Institute of Dermatology and Veneorology, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250022, China
  • Online:2020-06-15 Published:2020-06-04
  • Contact: TIAN Hongqing, E-mail: tianhq2006@126.com

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Abstract:

Objective: To determine the DNA gene types of Treponema Pallidum in the patients with syphilis in our hospital through multilocus sequence typing (MLST). Methods: Samples were collected from clinical lesions with cotton swabs and TP-DNA was extracted and confirmed by quantitative real-time PCR. The genetypes of TP were detected by MLST. Results: Fifty-five samples were detected and 9 genetypes were identified, including type 1.1.8 in 42 cases, type 3.2.3 in 5, type 1.1.1 in 2, type 24.1.8 in 1, type 25.1.8 in 1, type 1.52.8 in 1, type 1.53.8 in 1, type 1.1.17 in 1, type 25.3.1 in 1. Type 24.1.8, 25.1.8, 1.52.8, 1.53.8, 1.1.17 and 25.3.1 were reported firstly. All types of TP were resistant to macrolides. Conclusion: TP genetypes are variegated in the patients with syphilis in our hospital. The dominant type is 1.1.8, and all types of TP are resistant to macrolide.

Key words: Treponema Pallidum, multilocus sequence typing