China Journal of Leprosy and Skin Diseases ›› 2019, Vol. 35 ›› Issue (5): 280-283.doi: 10.12144/zgmfskin201905280

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Construction of infection model of Treponema pallidum strains clinically collected in rabbits

ZHANG Yuan1,2#, REN Zhuo1,2#, HOU Jianling2, ZHENG Rongtao2, LI Zhongwei2, LIU Jianxin2, PANG Jing2, HUANG Na2, TIAN Hongqing1,2   

  1. 1. School of Medicine and Life Sciences, University of Jinan－Shandong Academy of Medical Sciences, Jinan 250000, China; 2. Shandong Dermatology Hospital, Shandong Provincial Institute of Dermatology and Venereology, Jinan 250022, China #: equal contributiom
  • Online:2019-05-15 Published:2019-06-03
  • Contact: TIAN Hongqing, E-mail: thq2006@126.com

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Abstract: Objective: To establish the animal model of Treponema pallidum strain clinically collected. Methods: The clinically isolated Treponema pallidum strain was prepared for suspension. The suspension was injected into New Zealand rabbit and continuously passaged to next generations. The clinical symptoms of rabbit and serological changes were observed and tested. Results: A total of 6 strains of Treponema pallidum (A-F) were collected and 5 strains (A, C, D, E, F) were successfully inoculated. TPPA conversion was detected on days 28, 7, 14, 32 and 35, respectively. The strain A was passaged and the testicles showed swelling after inoculated strain A for 6 days and positive TPPA conversion after 7.7 days on average. Conclusion: The strain of Treponema pallidum can be successfully isolated clinically and passaged by rabbit testicular inoculation method.

Key words: syphilis, Treponema pallidum, animal model, rabbit