China Journal of Leprosy and Skin Diseases ›› 2022, Vol. 38 ›› Issue (8): 524-529.doi: 10.12144/zgmfskin202208524

• Original Articles • Previous Articles     Next Articles

Synergistic effect of EphB6 overexpression and BRAFi treatment in inhibiting the metastatic progression of melanoma

LIU Na1,2, LI Pin3, WANG Qixia4, LI Mingming5, HUANG Shuhong4, DANG Ningning5   

  1. 1 Weifang Medical University, Weifang 261000, China; 2 Central Hospital Affiliated to Shandong First Medical University, Jinan 251003, China; 3 Medical Science and Technology Innovation Center of Shandong First Medical University (Shandong Academy of Medical Sciences), Jinan 250117, China; 4 Preclinical Medicine School of Shandong First Medical University (Shandong Academy of Medical Sciences), Jinan 250117, China; 5 Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, China
  • Online:2022-08-15 Published:2022-06-22

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Abstract: Objective: To indicate the synergistic effect of EphB6 overexpression and BRAF inhibitor (BRAFi) treatment in inhibiting the metastatic progression of melanoma. Methods: The vemurafenib and dabrafenib targeted genes were downloaded from the Pubchem database, while the disease differentially expressed genes were analyzed from GSE141484 and GSE22838 chips, and the EphB6 was identified. The independent and synergistic effect EphB6 overexpression with BRAFi treatment on the viability and motility of A375 and 1205Lu cells were detected by using CCK-8, clone formation, wound-healing and transwell assays. Results: EphB6 overexpression significantly inhibited the proliferation, clone formation of A375 and 1205Lu cells, and potiently hindered the speed of cells through matrigel and wound healing. Furthermore, EphB6 overexpression promoted the suppressor effect of vemurafenib and dabrafenib on A375 and 1205Lu cells viability and motility. Conclusion: EphB6 overexpression significantly inhibited the metastatic progression of melanoma, and was beneficial to the BRAFi therapy of melanoma.

Key words: melanoma, BRAF inhibitor, EphB6, proliferation, invasion