Abstract: Objective: To investigate the effects of CXCL12 on the biological behavior of human melanoma M14 cell line. Methods: The human melanoma M14 cell line was incubated or induced by different concentrations of CXCL12 (0,10,50,100,200 ng/mL). The Cell Counting Kit-8 (CCK8), transwell migration and invasion assay were used to evaluate the proliferation, migration, and invasion of M14 cells. The Western blot was used to detect the protein expression of ERK, p-ERK, AKT, and p-AKT of M14 cells treated with 100 ng/mL CXCL12 at different times (0, 5, 10, 20, 30, 60 min). Results: CXCL12 promoted the proliferation, migration and invasion of M14 cells, and the activation of the ERK and AKT signaling pathways. Conclusion: CXCL12 may be associated with the progression of melanoma.

Key words: melanoma, CXCL12, tumor cell biological behavior