China Journal of Leprosy and Skin Diseases ›› 2017, Vol. 33 ›› Issue (5): 284-286.

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Effects of corn silk extracts on the migration of melanoma cell line A375

WU Jingying1, HUANG Jinmei2, LI Mingjiu3, GU Dong1.   

  1. 1. Xinsha Hospital, Nansha 511473, Guangzhou, China; 2. The Dermatology Institute of Guangdong, Guangzhou 510090, China; 3. The Chronic Disease Prevention and Treatment Station,Panyu District, Guangzhou 511400, China
  • Online:2017-05-15 Published:2018-12-19
  • Contact: WU Jingying, E-mail: gwc19880401@163.com

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Abstract:

Objective: To determine the effect and mechanisms of corn silk extracts on the migration of cutaneous melanoma. Methods: The skin melanoma cell line A375 was cultured and was treated by corn silk extracts in the concentration of 0.3, 3,30 μg/mL. The migration of A375 cells was detected by scratch-wound assay and the expression of migration related genes was detected by the Real time PCR. Results: The migration number of A375 cells in the group of corn silk extracts was less than that in the blank group and the higher the concentration, the less migration number was. Compared to the blank group, the expression level of EMT associated genes (TWIST1, SNAI1, SNAI2 and VIM) was lower and the expression level of PTGS2 was higher in the group of corn silk extracts. Conclusion: The corn silk extracts can inhibit the migration of the skin melanoma and increase the expression of EMT related genes.

Key words: corn silk extracts, melanoma, migration, EMT