Abstract: Objective: To evaluate the effect of Grifola frondosa polysaccharide (GFP) on the inflammatory factors of HaCaT cells induced by TNF-α / IFN-γ. Methods: The HaCaT cells induced by TNF-α / IFN-γ were used as the model of inflammation. After 24h of intervention, the expression of TARC, MDC, IL-6 and TNF-α mRNA，and protein in HaCaT cells were detected by qPCR and ELISA respectively. The expression of NF-κB and p-NF-κB were detected by Western blot. Results: The mRNA and protein expression of TARC, MDC and p-NF-κB were inhibited after intervened by GFP. Conclusion: GFP has the anti-inflammatory effect, which may be associated with the NF-κB P65 signaling pathway.

Key words: grifola frondosa polysaccharide, HaCaT cells, inflammatory factors, signaling pathways