China Journal of Leprosy and Skin Diseases ›› 2020, Vol. 36 ›› Issue (11): 663-666.doi: 10.12144/zgmfskin202011663

• Original Articles • Previous Articles     Next Articles

Longdanxiegan decoction activates Bax/Caspase-3 pathway and induces the apoptosis of HaCaT cells

YANG Guanqun1, GUO Chun1, WEI Haifeng2, ZHOU Yu1, ZHOU Shujie1, LIANG Chenxi1, WU Guojing1, XU Bohan1, ZOU Yongxin1, ZHANG Xiaojie3, SUN Shuna3   

  1. 1 Department of Genetics, School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong University, Jinan 250012, China;
    2 Shandong University of Traditional Chinese Medicine, Jinan 250355, China; 
    3 Department of Dermatology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250011, China
  • Online:2020-11-15 Published:2020-10-30
  • Contact: SUN Shuna, E-mail: 445178992@qq.com

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Abstract: Objective: To determine the role of Longdanxiegan decoction in the apoptosis of HaCaT cells. Methods: The effect of Longdanxiegan decoction on the apoptosis of HaCaT cells was detected by Tunel method. The apoptosis of HaCaT cells was detected by fluorescence microscopy after DAPI staining. The effect of HaCaT cells on the expression of apoptosis-related proteins caspase-3, Bcl-2 and Bax was detected by Western blot. Results: The abnormal cells shape rate and the apoptosis rate were higher in the Longdanxiegan decoction group than in the untreated group. Longdanxiegan decoction significantly down-regulated the expression level of Bcl-2 and up-regulated Bax, and actived caspase in HaCaT cells. The cell apoptosis caused by Longdanxiegan decoction decreased in the cells pretreated by Z-DEVD-FMK (inhibitor of caspase-3). Conclusion: Longdanxiegan decoction can significantly induce apoptosis of human keratinocytes, which may be through down-regulation of Bcl-2 and up-regulation of caspase-3 or Bax.

Key words: Longdanxiegan decoction, HaCaT cells, psoriasis, apoptosis