Abstract: Objective: To investigate the difference of probiotics in the intestinal flora between children with atopic dermatitis （AD group） and normal children (N group). Method: The high-throughput sequencing of the 16SrRNA gene V3-V4 region of the fecal bacteria in AD group and normal control group was detected by the Illumina MiSeq sequencing platform. The difference of probiotics in the levels of the genus and species were compared. Result: Thirty five children AD and 27 normal controls were recruited. At the level of the genus, the species abundance of Bifidobacterium in AD group was higher than that in the N group, the species abundance of Lactobacillus was lower than that in N group, but there was no significant differences between them (P>0.05). At the level of the species, compared to the N group, the abundance of Bifidobacterium pseudocatenulatum_DSM_20438__JCM_1200__LMG_10505, unclassified_g__Bifidobacterium, Bifidobacterium_adolescentis and Bifidobacterium_animalis in the AD group was higher and the Bifidobacterium_breve species abundance was lower, with no significant difference (P> 0.05). The Lactobacillus_salivarius species abundance of AD group was lower than that in N group, with no significant difference (P>0.05). The species abundance of the unclassified_g_ Lactobacillus, Lactobacillus_plantarum_subsp_plantarum of AD group was higher than that in N group, with significant difference (P< 0.05). Conclusion:The abundance of probiotic species in the intestinal flora of children with atopic dermatitis is different from that of normal children.

Key words: atopic dermatitis, intestinal flora, probiotics