Abstract: Objective: To determine the effect of linoleic acid-ceramide-containing（LA-Cer） moisturizer on inflammation and barrier in atopic dermatitis (AD) mouse model. Methods: Eighteen BALB/c mice were divided into the disease group, treatment group and control group, 6 mice in each group. The ears of mice in the treatment group and disease group were given calcipotriol topically for 2 weeks to induced AD model. The mice in the treatment group were given additional LA-Cer containing moisturizer twice daily. The mice in the control group were given topical absolute ethyl alcohol. The differences of clinical phenotype, histopathology, and stratum corneum hydration (SC Hydration) were observed in the three groups. The level of the inflammation-related cytokines (TSLP，IL-4，IL-10，IL-1β，IL-33 and IFNγ ) were detected by real-time quantitative PCR. Results: The AD model was constructed successfully. The ear thickness and SC hydtarion in the treatment group were (0.375±0.015)cm and (22.500±2.081)au, while in disease group were (0.510±0.035) cm and with (6.750±1.258)au, with significant differences (Ps<0.05). The level of inflammatory factors TSLP and IL-1β of the lesions in the treatment group were lower than those in the disease group. Conclusion: Topical LA-Cer containing moisturizer can reduce the inflammation and repair the skin barrier in a mouse model of atopic dermatitis induced by calcipotriol.

Key words: atopic dermatitis, skin barrier, moisturizer