中国麻风皮肤病杂志 ›› 2023, Vol. 39 ›› Issue (7): 501-504.doi: 10.12144/zgmfskin202307501

• 论著 • 上一篇    下一篇

氢气对HaCaT角质形成细胞增殖及炎症因子表达的影响

郭淑芬,吴嘉惠,刘伯言,陈文文,耿彪,刘志超   

  1. 山东第一医科大学第二附属医院，泰安，271000
  • 出版日期:2023-07-15 发布日期:2023-07-05

Effects of hydrogen on proliferation of HaCaT keratinocytes and expression of inflammatory factors

GUO Shufen, WU Jiahui, LIU Boyan, CHEN Wenwen, GENG Biao, LIU Zhichao   

  1. The Second Affiliated Hospital of Shandong First Medical University, Taian 271000, China
  • Online:2023-07-15 Published:2023-07-05

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摘要： 目的：明确氢气（H2）对银屑病角质形成细胞模型增殖及炎症因子表达的影响，为探索氢气治疗银屑病的可能机制提供参考。方法：采用M5（含IL-17A、IL-22、肿瘤抑制素M、IL-lα和TNF-α）诱导HaCaT角质形成细胞过度增殖，并通过氢气细胞培养箱进行氢气干预。将HaCaT细胞分为Control组（无任何干预）、Model组（2.5 ng/mL M5处理）、H2组（2.5 ng/mL M5、25%浓度的氢气处理）、Acitretin组（2.5 ng/mL M5、10 μmol/L的阿维A处理作为阳性对照），72小时后CCK-8法对细胞增殖进行测定，qRT-PCR检测细胞过度增殖标记因子KRT6及炎症因子IL-1β、IL-6、TNF-α的mRNA表达水平。结果：CCK-8法检测结果显示不同浓度氢气对正常细胞生长无影响，25%浓度的氢气干预能显著抑制M5诱导的细胞增殖，降低KRT6及IL-1β、IL-6、TNF-α的mRNA表达。结论：氢气干预能够抑制M5诱导的HaCaT细胞的过度增殖，降低炎症因子的表达。

关键词: 氢气, HaCaT细胞, 增殖, 炎症因子, 银屑病

Abstract: Objective: To determine the effect of hydrogen (H2) on the hyperproliferation of HaCaT keratinocytes and the expression of inflammatory factors, and to explore the possible mechanism of hydrogen in the treatment of psoriasis. Methods: Hyperproliferation of HaCaT keratinocytes was induced by M5 (IL-17A, IL-22, tumor inhibin -M, IL-1α And TNF-α）and hydrogen intervention was conducted through hydrogen cell incubator. HaCaT cells were divided into Control group (without any intervention), Model group (treated by 2.5 ng/mL M5), H2 group (treated by 2.5 ng/mL M5 and 25% hydrogen treatment), and Acitretin group (treated by 2.5 ng/mL M5, 10 μMol/L of acitretin A), after 72 hours, the cell viability was determined by CCK-8 method, and the cell proliferation marker KRT6 and inflammatory factor IL-1β, IL-6,TNF-α mRNA expression level were detected by qRT-PCR. Results: CCK-8 assay showed that different concentrations of hydrogen had no effect on normal cell growth. Hydrogen intervention at 25% concentration can significantly inhibit the enhancement of cell viability induced by M5, and reduced the level of KRT6 and IL-1β, IL-6, and NF-αmRNA expression. Conclusion: Hydrogen intervention can inhibit the over proliferation of HaCaT cells induced by M5 and reduce the expression of inflammatory factors.

Key words: hydrogen, HaCaT cells, proliferation, inflammatory cytokines, psoriasis