中国麻风皮肤病杂志 ›› 2025, Vol. 41 ›› Issue (5): 330-336.doi: 10.12144/zgmfskin202505330

• 论著 • 上一篇    下一篇

慢性自发性荨麻疹患者外周血淋巴细胞ICOS/ICOSL表达及与免疫细胞的相关性研究

胡颖1,邓丽钧2,陈佳璐1,焦晴晴1   

  1. 1苏州大学附属第一医院皮肤性病科，江苏苏州，215006； 2苏州市吴中人民医院皮肤科,江苏苏州,215128
  • 出版日期:2025-05-15 发布日期:2025-05-14

Expression of ICOS/ICOSL lymphocyte in peripheral blood of the patients with chronic spontaneous urticaria and its correlation with immune cells

HU Ying1, DENG Lijun2, CHEN Jialu1, JIAO Qingqing1   

  1. 1 Department of Dermatology, the First Affiliated Hospital of Soochow University, Suzhou 215006, China; 2 Department of Dermatology, Suzhou Wuzhong People's Hospital, Suzhou 215128, China
  • Online:2025-05-15 Published:2025-05-14

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摘要： 目的：明确慢性自发性荨麻疹（chronic spontaneous urticaria，CSU）患者外周血中Th1和Treg细胞的表达情况，观察诱导性共刺激分子（ICOS）及其配体（ICOSL）信号与外周血T/B细胞之间的关系。方法：采用流式细胞术分析外周血Th1、Treg细胞及其表面ICOS的表达情况，并对B细胞表面ICOSL进行鉴定。结果：患者ICOS+ Th1细胞和ICOS+ Treg细胞比例分别为（15.19±21.96）%和（60.04±31.82）%，显著高于对照组的（2.24±2.62）%和（48.72±19.13）%，差异均有统计学意义（均P＜0.05）。Th1，ICOS+ Th1与嗜酸性粒细胞均呈正相关（r=0.4998,0.4701, 均P＜0.05），ICOS+ Treg细胞与中性粒细胞呈负相关(r=-0.3771，P＜0.05)。此外，Th1细胞与CD19+ B细胞以及ICOS+ Th1与CD19+ICOSL+ B细胞均呈正相关（r=0.5218,0.5616, 均P＜0.05），而ICOS+ Treg细胞与CD19+ B细胞、CD19+ICOSL+ B细胞均呈负相关（r=-0.4183,-0.4519,均P＜0.05）。结论：CSU患者外周血中ICOS+ Th1和ICOS+ Treg细胞比例显著增加，且与嗜酸性粒细胞、中性粒细胞和CD19+ICOSL+ B细胞之间存在相关性，提示了ICOS/ICOSL通路在CSU免疫调节中的潜在作用。

关键词: 慢性自发性荨麻疹, Th1细胞, Treg细胞, ICOS/ICOSL, B细胞

Abstract: Objective: To determine the expression of Th1 and Treg cells in patients with chronic spontaneous urticaria (CSU) and observe the role of ICOS/ICOSL signaling between peripheral blood T/B cells in CSU. Methods: The expression of Th1, Treg cells, and surface ICOS in peripheral blood, and surface ICOSL on B cells was analyzed by flow cytometry. Results: The proportions of ICOS+ Th1 cells and ICOS+ Treg cells in patients were (15.19±21.96)% and (60.04±31.82)% respectively, significantly higher than those in the control group, which were (2.24±2.62)% and (48.72±19.13)% respectively, and the differences were statistically significant (both P＜0.05). Th1 and ICOS+ Th1 cells were positively correlated with eosinophils (r=0.4998, 0.4701, Ps＜0.05), and ICOS+ Treg cells were negatively correlated with neutrophils (r=-0.3771, P＜0.05). In addition, Th1 cells were positively correlated with CD19+ B cells, and ICOS+ Th1 cells were positively correlated with CD19+ICOSL+ B cells (r=0.5218, 0.5616, Ps＜0.05), while ICOS+ Treg cells were negatively correlated with CD19+ B cells and CD19+ICOSL+ B cells (r=-0.4183, -0.4519, Ps＜0.05). Conclusion: The proportions of ICOS+ Th1 and ICOS+ Treg cells in the peripheral blood of CSU patients are significantly increased, and their correlation with eosinophils and neutrophils as well as CD19+ICOSL+ B cells suggests a potential role of the ICOS/ICOSL pathway in the immune regulation of CSU.

Key words: chronic spontaneous urticaria, Th1 cell, Treg cell, ICOS/ICOSL, B cell