中国麻风皮肤病杂志 ›› 2016, Vol. 32 ›› Issue (11): 653-656.

• 论著 • 上一篇    下一篇

CD4＋CD25＋调节性T细胞与Th17细胞在寻常型银屑病中的表达

张玉丽1 ，王逸飞2 ，张春敏3   

  1. 1.聊城市人民医院，山东聊城，252000 2.山东大学医学院，山东济南，250014 3.山东大学第二医院，山东济南，250033
  • 出版日期:2016-11-15 发布日期:2018-12-20
  • 通讯作者: 张春敏，E-mail: cmzhang1878@163.com

Role of CD4+CD25+ regulatory T cells and Th17 cells in patients with psoriasis vulgaris

ZHANG Yuli1,WANG Yifei2,ZHANG Chunmin3   

  1. 1.Liaocheng People’s Hospital, Liaocheng 252000, China; 2. Shandong University School of Medicine, Jinan 250014, China; 3. The Second Hospital of Shandong University, Jinan 250033, China
  • Online:2016-11-15 Published:2018-12-20
  • Contact: ZHANG Chunmin, E-mail: cmzhang1878@163.com

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摘要： 目的：检测外周血中CD4＋CD25＋调节性T细胞（简称Treg细胞）与Th17细胞在寻常型银屑病（psoriasis vulgaris, PV) 中的表达。方法：34例寻常型银屑病患者，18例正常人作为对照。采用流式细胞术检测外周血中Treg细胞、FOXP3+Treg细胞及Th17细胞的表达水平。结果：PV患者外周血中Treg细胞比例和FOXP3+Treg细胞比例分别为（3.68±1.22）%和（0.53±0.19）%，低于正常对照组的（6.63±1.00）% 和（0.76±0.14）% (P﹤0.05)。Th17细胞比例为（2.20±0.78）%，高于正常对照组的（0.65±0.22）% (P﹤0.05)。PV患者外周血中Treg细胞的表达与FOXP3+Treg细胞的表达呈正相关（r=0.563，P<0.05），而Treg细胞的表达与Th17细胞的表达呈负相关（r=-0.522，P<0.05）；Treg细胞和Th17细胞二者与PASI评分无相关性，与病程亦无相关性。结论：Treg细胞表达的减少及Th17细胞应答的增强，可能是导致PV免疫失衡的重要原因。

关键词: 寻常型银屑病, CD4+CD25+调节性T细胞, Th17细胞, Foxp3, PASI评分, 流式细胞术

Abstract: Objective: To detect the expression of Treg cells and Th17 cells in the patients with psoriasis vulgaris（PV）. Methods: Thirty-four PV and 18 healthy controls were enrolled in the study, and Treg cells, Foxp3 and Th17 cells of the blood were detected by flow cytometry. Results: The percentage of Treg cells and Foxp3 cells were（3.68±1.22）% and （0.53±0.19）%, which were significantly lower than those in normal controls（6.63±1.00）%, and （0.76±0.14）% (P﹤0.05). The percentage of Th17 cells was（2.20±0.78）%, which was significantly higher than that in normal controls （0.65±0.22）% (P﹤0.05). The expression of Treg cells and FOXP3+Treg cells was positively correlated (r=0.563, P﹤0.05), but the expression of Treg cells and Th17 cells was negatively correlated (r=-0.522, P﹤0.05). The correlation among Treg cells, Th17 cells, PASI score and duration of the disease was not significant (P﹥0.05).. Conclusion: The decrease in the expression of Treg cells and the increased response of Th17 cells may be the important factors for the immunological?imbalance in PV.

Key words: psoriasis vulgaris, regulatory T cells;Th17 cells, FOXP3, PASI score, flow cytometry