白芍总苷,特应性皮炎,细胞因子,小鼠 ," /> 白芍总苷,特应性皮炎,细胞因子,小鼠 ,"/> total glucosides of paeony,atopic dermatitis,cytokines,mice

  ,"/> <p class="MsoNormal"> <span>白芍总苷对</span>Nc/Nga<span>小鼠特应性皮炎的保护作用</span>

中国麻风皮肤病杂志 ›› 2017, Vol. 33 ›› Issue (1): 31-33.

• 论著 • 上一篇    下一篇

白芍总苷对Nc/Nga小鼠特应性皮炎的保护作用

刘斌1  李静1  胡惠清1  朱里2   

  1. 1武汉市第九医院皮肤科,430080

    2华中科技大学同济医学院附属协和医院皮肤科,武汉，430030

  • 出版日期:2017-01-15 发布日期:2018-12-11
  • 通讯作者: 朱里，E-mail: wqlb3344@163.com

Effect of the total glucosides of paeony on experimental atopic dermatitis in mice

IU Bin1, LI Jing1, HU Huiqing1, ZHU Li2   

  1. 1.Department of Dermatology, The Ninth Hospital of Wuhan City, Wuhan 430080, China; 2.Department of Dermatology, Hua Zhong University of Science and Technology, Union Hospital of tongji Medical College，Wuhan 430030, China

  • Online:2017-01-15 Published:2018-12-11
  • Contact: ZHU Li, E-mail: wqlb3344@163.com

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摘要：

目的：确定白芍总苷(Total Glucosides of Paeony,TGP)对小鼠特应性皮炎的保护作用。方法：利用2,4-二硝基氯苯(DNCB)建立Nc/Nga小鼠实验性特应性皮炎模型。30只小鼠分为3组：正常对照组，模型组，实验组（TGP治疗），每组10只。实验组连续灌胃用药2周，分别于第8 d、15 d测量各组小鼠背部皮损厚度和皮肤质量，并利用ELISA法分别检测第8 d、15 d血清中细胞因子IFN-γ，IL-4,IL-5和IgE的表达。结果：白芍总苷治疗第8 d、15 d，皮损厚度变薄及皮损皮肤质量明显减小（P＜0.05），血清细胞因子IL-4、IL-5和IgE水平明显低于模型组，IFN-γ水平明显高于模型组（P＜0.05）。结论：白芍总苷可能通过促进IFN-γ分泌，下调炎症因子IL-4、IL-5水平以及抑制IgE表达从而对小鼠特应性皮炎产生保护作用。

关键词: 白芍总苷')">

白芍总苷, 特应性皮炎, 细胞因子, 小鼠

Abstract:

Objective: To determine the effect of total glucosides of paeony (TGP) on atopic dermatitis in mice.  Methods: An atopic dermatitis model was established on the back of NC/Nga mice with DNCB. Thirty mice were divided into 3 groups: control group, model group and TGP group (10 mice in each group). The mice of TGP group were treated by TGP for 14 days. The levels of serum cytokines including IL-4 and IL-5, IFN-γ and IgE were detected with ELISA kit at day 8th and 15th. Results: TGP could significantly decrease the skin thickness and weightness at 8 days and 15 days (P＜0.05). The levels of IL-4,IL-5 and IgE were significantly decreased , while the level of IFN-γ was increased in TGP group, compared with other groups. Conclusion: TGP has protective effects on atopic dermatitis in mice, which may be through inhibiting production of IgE, IL-4 and IL-5, and promoting the secretion of IFN-γ.

Key words: total glucosides of paeony')">

total glucosides of paeony, atopic dermatitis, cytokines,   ')">mice