mTORC1信号通路在狼疮鼠体内的表达及意义

中国麻风皮肤病杂志 ›› 2018, Vol. 34 ›› Issue (8): 449-452.

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mTORC1信号通路在狼疮鼠体内的表达及意义

谢双德¹ 魏姗姗² 张华年¹王桂松¹ 彭学标¹赵珂³ 李俊灏¹ 赖宽

1南方医科大学南方医院皮肤科，广州，510515 2南方医科大学珠江医院皮肤科，广州，510282 3南方医科大学南方医院血液科，广州，510515

出版日期:2018-08-15 发布日期:2018-12-11
通讯作者: 赖宽，E-mail：kuan_lai@163.com

The expression of mTORC1 pathway in murine model of systemic lupus erythematosus

XIE Shuangde¹，WEI Shanshan², ZHANG Huanian¹, WANG Guisong¹, PENG Xuebiao1, Zhao Ke³, LI Junhao¹，LAI Kuan¹

1.Departmen of Dematology,Nanfang Hospital，Southern Medical University,Guangzhou 510515，China;2.Departmen of Dematology,Zhujiang Hospital,Southern Medical University，Guangzhou 510282，China;3.Department of Hematology,Nanfang Hospital，Southern Medical University，Guangzhou 510515, China

Online:2018-08-15 Published:2018-12-11
Contact: LAI Kuan, E-mail:kuan_lai@163.com

摘要/Abstract

摘要： 目的：检测mTORC1信号通路在系统性红斑狼疮鼠体内的表达，探讨其在系统性红斑狼疮发病中的作用。方法：研究对象为6只B6.MRL/lpr狼疮小鼠和6只C57正常小鼠。RT-PCR和Western blot检测肾脏组织Akt、mTOR、p70S6K、IL-17 mRNA和蛋白的表达。细胞内染色流式细胞术检测脾脏Th17淋巴细胞亚群比例。结果：狼疮鼠组Akt、mTOR、p70S6K、IL-17 mRNA、磷酸化蛋白表达水平高于对照组（P<0.05）；狼疮鼠组的Th17细胞占CD4+T细胞的比例高于正常对照组（P<0.05）。结论： mTORC1（Akt-mTOR-p70S6K）信号通路在SLE的发病中起着重要作用。

关键词: 系统性红斑狼疮, mTOR, p70S6K, Th17

Abstract: Objective: To detect the level of mTORC1 pathway related factors in the murine model of systemic lupus erythematosus (SLE) and to explore its effect in the development of SLE. Methods: There were 6 B6.MRL/lpr lupus mice in the experimental group and 6 C57 mice in the control group. The levels of Akt, mTOR, p70S6K, IL-17 mRNA and protein in kidney were detected by RT-PCR and western blot. The percentage of Th17 lymphocytes in CD4+T cells in spleen was detected by flow cytometry. Results: The mRNA and phosphoprotein of Akt, mTOR, p70S6K and IL-17 in the experimental group were higher than those in the control group. The percentage of Th17 lymphocytes in CD4+T cells in the experimental group was higher than that in the control group. Conclusion: The activation of mTORC1(Akt-mTOR-p70S6K) pathway may play an important role in the development of SLE.

Key words: systemic lupus erythematosus, mTOR, p70S6K, Th17

谢双德, 魏姗姗, 张华年, 王桂松, 彭学标, 赵珂, 李俊灏, 赖宽. mTORC1信号通路在狼疮鼠体内的表达及意义[J]. 中国麻风皮肤病杂志, 2018, 34(8): 449-452.

XIE Shuangde, WEI Shanshan, ZHANG Huanian, WANG Guisong, PENG Xuebiao, Zhao Ke, LI Junhao, LAI Kuan. The expression of mTORC1 pathway in murine model of systemic lupus erythematosus[J]. China Journal of Leprosy and Skin Diseases, 2018, 34(8): 449-452.

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mTORC1信号通路在狼疮鼠体内的表达及意义

The expression of mTORC1 pathway in murine model of systemic lupus erythematosus

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