中国麻风皮肤病杂志 ›› 2018, Vol. 34 ›› Issue (9): 546-549，554.

• 短篇论著 • 上一篇    下一篇

Toll样受体9基因多态性与湖北汉族系统性红斑狼疮易感性的关系

邢亚琼，吴雪菁子，宋继权   

  1. 武汉大学中南医院皮肤科，湖北武汉，430071
  • 出版日期:2018-09-15 发布日期:2018-12-11
  • 通讯作者: 系统性红斑狼疮；Toll样受体9；单核苷酸多态性

Association between Toll like receptor 9 gene polymorphism and susceptibility to systemic lupus erythematosus in Hubei Han population

XING Yaqiong , WU Xuejingzi , SONG Jiquan   

  1. Department of Dermatology of Zhongnan Hospital of Wuhan University ,Wuhan 430071, China
  • Online:2018-09-15 Published:2018-12-11
  • Contact: Systemic lupus erythematosus; Toll like receptor 9; single nucleotide polymorphism

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摘要： 目的：明确Toll样受体9基因rs187084、rs352140位点单核苷酸多态性与湖北汉族人口中系统性红斑狼疮疾病易感性的关系。方法：PCR扩增80例系统性红斑狼疮患者和84例健康志愿者的外周血目的基因片段，通过SNaPshot法检测rs187084、rs352140这两个位点的基因型及等位基因频率。结果：系统性红斑狼疮实验组及健康对照组中rs187084的AA,GG,AG基因型频率分别为37.50%，18.75%，43.75%和47.62%，13.10%，39.29%，无显著统计学差异（P>0.05）。rs352140 AA,GG,AG基因型频率在实验组及对照组中分别为18.75%，43.75%，37.50%和11.90%，48.81%，39.29%，无显著统计学差异（P>0.05）。结论：Toll样受体9的rs187084、rs352140基因位点的单核苷酸多态性可能与系统性红斑狼疮的发病不相关。

关键词: 系统性红斑狼疮, Toll样受体9, 单核苷酸多态性

Abstract: Objective: To determine the association between single nucleotide polymorphisms of Toll like receptor 9 related gene loci and systemic lupus erythematosus (SLE) in Hubei Han population. Methods: Gene fragment of the peripheral blood from 80 patients with systemic lupus erythematosus and 84 healthy controls was amplified by PCR. The genotype and allele frequency of the two loci of rs187084 and rs352140 was detected by SNaPshot. Results: The AA, GG, and AG genotype frequencies of rs187084 in the patients group and healthy control group were 37.50%, 18.75%, 43.75% and 47.62%, 13.10%, 39.29%, respectively, with no significant differences (Ps>0.05). The genotype frequencies of rs352140 AA, GG, and AG in the patients group and healthy control group were 18.75%, 43.75%, 37.50% and 11.90%, 48.81%, 39.29%, respectively, with no significant differences (Ps>0.05). Conclusion: The single nucleotide polymorphisms of rs187084 and rs352140 loci in Toll like receptor 9 may not be associated with the pathogenesis of SLE.

Key words: Systemic lupus erythematosus, Toll like receptor 9, single nucleotide polymorphism

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