中国麻风皮肤病杂志 ›› 2025, Vol. 41 ›› Issue (9): 663-669.doi: 10.12144/zgmfskin202509663

• 论著 • 上一篇    下一篇

海分枝杆菌感染小鼠模型的构建

林秀球1,2,李阳2,佘昕妍2,关则璞2,王晓华2   

  1. 1深圳市慢性病防治中心，深圳，518000; 2南方医科大学皮肤病医院，广东广州，510091
  • 出版日期:2025-09-15 发布日期:2025-09-08

Construction of a mouse model infected by Mycobacterium marinum

LIN Xiuqiu1,2, LI Yang2, SHE Xinyan2, GUAN Zepu2, WANG Xiaohua2   

  1. 1 Shenzhen Center for Chronic Disease Control, Shenzhen 518000, China; 2 Dermatology Hospital of Southern Medical University, Guangzhou 510091, China
  • Online:2025-09-15 Published:2025-09-08

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摘要： 目的：构建稳定可重复的海分枝杆菌(Mycobacterium marinum, M. marinum)局部感染小鼠模型，模拟人类皮肤及软组织感染病理生理特征。方法：通过小鼠背部皮下/足垫注射法接种菌株（菌量分别为106 CFU、104 CFU、102 CFU），按照不同饲养环境（18℃和25℃），动态监测感染局部病变、组织病理及免疫反应。结果：成功构建小鼠局部海分枝杆菌肉芽肿炎症模型，感染严重程度呈菌量依赖性。低温早期促进低菌量组皮肤感染肉芽肿形成。与足垫组相比，皮肤组感染范围更局限。M. marinum不易扩散，内脏未见感染。结论：本研究建立的局部感染小鼠模型具有可重复性强，病理特征明确，为海分枝杆菌致病机制研究及抗分枝杆菌药物开发提供了重要工具。

关键词: 海分枝杆菌, 小鼠, 皮肤感染模型, 足垫感染模型

Abstract: Objective: To construct a stable and repeatable mouse model of local infection of Mycobacterium marinum (M. marinum) and simulate the pathophysiological characteristics of human skin and soft tissue infection. Methods: The strains were inoculated by subcutaneous/pad injection on the back of mice (with bacterial amounts of 106CFU, 104CFU, and 102CFU respectively), and the local lesions, histopathology and immune responses of the infection were dynamically monitored according to different rearing environments (18℃ and 25℃). Results: The inflammation model of local M. marinum granuloma was successfully established in mice, and the severity of infection was dose-dependent. Low temperature promoted early formation of skin infection granuloma in low dose group. Compared with the foot pad group, the infection scope of the skin group was more limited, M. marinum was not easy to spread, and no infection was found in the internal organs. Conclusion: The mouse model of local infection established in this study has strong repeatability and clear pathological characteristics, which provides an important tool for the study of the pathogenic mechanism of M. marinum and the development of anti-mycobacterium drugs.

Key words: Mycobacterium marinum, mice, skin infection model, foot pad infection model