中国麻风皮肤病杂志 ›› 2016, Vol. 32 ›› Issue (6): 327-330.

• 论著 • 上一篇    下一篇

氧化苦参碱对人黑素细胞增殖与炎症因子自分泌的影响

罗 宏 范英姬 王海波 王 娟 张 艳 杜 倩   

  1. 郴州市第三人民医院，423000
  • 出版日期:2016-06-15 发布日期:2018-12-25
  • 通讯作者: 罗宏，E-mail: 3602677@qq.com

Influence of oxymatrine on the melanocyte proliferation and inflammation factor autocrine

LUO Hong, FAN Yingji, WANG Haibo, WANG Juan, ZHANG Yan, DU Qian.   

  1. Chenzhou 3rd People’s Hospital,Chenzhou 423000,China
  • Online:2016-06-15 Published:2018-12-25
  • Contact: LUO Hong, E-mail: 3602677@qq.com

PDF (PC)

124

摘要： 目的：明确氧化苦参碱(OMT)对黑素细胞增殖、TLR3、NF-κB的mRNA表达及相关细胞因子的影响。方法：人表皮黑素细胞传代培养，分为空白对照组及不同浓度的氧化苦参碱组（10 μmol/L，50 μmol/L和100 μmol/L），用MTT法测定细胞增殖，RT-qPCR检测TLR3、NF-κB的mRNA表达，ELISA试剂盒检测IL-6、IL-8及IL-17表达。结果：各组黑素细胞的吸光度值均显著高于空白对照组，随OMT浓度上升而升高，当OMT浓度为50 μmol/L时增殖率达到平台期。OMT组TLR3及NF-κB mRNA 及IL-6、IL-8及IL-17蛋白表达较对照组显著降低，差异有统计学意义 (P<0.05)。结论： 氧化苦参碱可促进黑素细胞的增殖，下调TLR3、NF-κB的mRNA表达，减少IL-6、IL-8及IL-17的生成。

关键词: 氧化苦参碱, 黑素细胞, TLR3, NF-κB

Abstract: Objective: To determine the influence of oxymatrine (OMT) on the melanocyte proliferation and inflammation factor autocrine. Methods: The cultured melanocytes were divided into blank group and oxymatrine groups with different concentrations (5 mg/mL, 10mg/mL and 15mg/mL). The proliferation of melanocyte, mRNA of TLR3 and NF-κB and the expression of IL-6, IL-8 and IL-17 were detected by MTT method, RT-qPCR and ELISA respectively. Result: Absorbance values of the melanocytes in oxymatrine groups were higher than that in control group, in a concentration-dependent manner. The proliferation rate reached a plateau when OMT concentration reached up to 50μmol/L. The expression of TLR3 and NF-κB mRNA and the proteins of IL-6, IL-8, IL-17 in OMT groups were significantly lower than those in control group（P<0.05). Conclusion: OMT can increase the proliferation of melanocyte, down-regulate the expression of TLR3 and NF-κB mRNA and the protein of IL-6, IL-8 and IL-17.

Key words: oxymatrine, melanocyte, TLR3, NF-κB, cytokines