Abstract: Two cases with orf were reported. The diagnosis was confirmed by clinical and laboratory fluorescence PCR detection. Case one, a 55-year-old female, presented with soybean-sized infectious granuloma on her left fourth finger for 1 month and multiple verrucous lesions on the vulva for 20 days. Case two, a 46-year-old female, presented with red nodules with tenderness for half month. Both patients had a contact history with infected sheep and both were positive for orf virus DNA. The lesions were gradually subsided after antiviral drug and cryotherapy.

Key words: orf, fluorescence PCR detection