Abstract:

Objective: To determine the optimal dose of ALA-PDT on the inhibition of A431 cells proliferation in cutaneous squamous cell carcinoma. Methods: A431 cells were cultured firstly in vitro, and then, were treated with different drug concentration (0.5, 1 and 2 mmol/L) and different dose of PDT (5, 10, 20 and 40 J/cm2). The proliferation of treated A431 cells was detected by MTT. Results: The inhibition rates of A431 cells treated under the condition of 0.5 mmol/L ALA combined with 5, 10, 20 and 40 J/cm2 PDT were 6%, 10%, 15% and 18% respectively. The inhibition rates of A431 cells treated with 1 mmol/L ALA combined with PDT of 5, 10, 20 and 40 J/cm2 PDT were 30%, 52%, 80% and 82% respectively. The inhibition rates of A431 cells treated with 2 mmol/L ALA combined with 5, 10, 20 and 40 J/cm2 PDT were 31%, 54%, 81% and 82% respectively. Conclusion: The highest inhibition rate of PDT on A431 cell proliferation is 1mmol/L ALA and 20 J/cm2 PDT

Key words: ALA-PDT')">

ALA-PDT, cutaneous squamous cell carcinoma, A431 cell, proliferatio