中国麻风皮肤病杂志 ›› 2023, Vol. 39 ›› Issue (5): 327-331.doi: 10.12144/zgmfskin202305327

• 论著 • 上一篇    下一篇

TLR4和CXCR4在天疱疮患者中的表达

陈艳梅,王川,刘亭亭,糜自豪,孙乐乐,赵晴,王娜,李丽宸,刘红,张福仁   

  1. 山东第一医科大学附属皮肤病医院(山东省皮肤病医院),山东省皮肤病性病防治研究所,济南,250022
  • 出版日期:2023-05-15 发布日期:2023-05-16

Expression of TLR4 and CXCR4 in patients with pemphigus

CHEN Yanmei, WANG Chuan, LIU Tingting, MI Zihao, SUN Lele, ZHAO Qing, WANG Na, LI Lichen, LIU Hong, ZHANG Furen   

  1. Shandong Provincial Hospital for Skin Diseases & Shandong Provincial Institute of Dermatology and Venereology, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250022, China
  • Online:2023-05-15 Published:2023-05-16

摘要: 目的:检测TLR4和CXCR4在天疱疮患者外周血及皮损中的水平,分析其在天疱疮病理机制中的潜在作用。方法:收集15例治疗前天疱疮患者和13名健康正常人的皮损组织及PBMC,通过免疫组化染色检测TLR4和CXCR4在皮肤中的表达情况,通过流式细胞仪检测PBMC中TLR4+CXCR4+浆细胞的比例。结果:天疱疮患者皮损部位TLR4和CXCR4表达增加;同时天疱疮患者PBMC中浆细胞(Lin-CD27+CD38+CD138+)的表达比例(3.26±1.90)高于健康对照(1.38±0.89),差异有统计学意义(P<0.05);进一步分析发现天疱疮患者浆细胞中TLR4+CXCR4+双阳细胞表达比例(14.23±8.69)高于健康对照(6.74±2.95),差异有统计学意义(P<0.05)。结论:天疱疮患者中TLR4以及CXCR4表达显著增加,可能在疾病病理进程中起到促进作用。

关键词: 天疱疮, 浆细胞, TLR4, CXCR4

Abstract: Objective: To detect the expression of TLR4 and CXCR4 in peripheral blood and skin lesions of patients with pemphigus, and to analyze their potential role in the pathological mechanism of pemphigus. Methods: The peripheral blood mononuclear cells (PBMCs) and skin lesions were collected from 15 patients with pemphigus before treatment and 13 healthy controls. The expression of TLR4 and CXCR4 were detected in skin by immunohistochemical (IHC) staining. Meanwhile, the frequencies of TLR4+CXCR4+ plasma cells (PCs) were detected by flow cytometry. Results: The expression of TLR4 and CXCR4 increased in the lesions of patients with pemphigus. The frequencies of circulating Lin-CD27+CD38+CD138+PC (3.26±1.90) in patients was high than that in health controls (1.38±0.89), with a significant difference (P<0.05). The frequencies of circulating TLR4+CXCR4+ plasma cells (PCs) (14.23±8.69) was high than that in health controls  (6.74±2.95), with a significant difference (P<0.05). Conclusion: The expression of TLR4 and CXCR4 in pemphigus are significantly high, which may play a promoting role in the pathological process of the disease.

Key words: pemphigus, plasma cell, TLR4, CXCR4