中国麻风皮肤病杂志 ›› 2024, Vol. 40 ›› Issue (6): 406-411.doi: 10.12144/zgmfskin202406406

• 论著 • 上一篇    下一篇

SLC35E基因家族在麻风皮损中的表达

许宗严1*,李阳2*,林秀球2,林俊杰2,王晓华2   

  1. 1深圳市福田区慢性病防治院，广东深圳，518000；2南方医科大学皮肤病医院，广东广州，510091
    *为共同第一作者
  • 出版日期:2024-06-15 发布日期:2024-05-07

Expression of SlC35E family in leprosy lesions

XU Zongyan1*, LI Yang2*, LIN Xiuqiu2, LIN Junjie2, WANG Xiaohua2   

  1. 1 Futian Center For Chronic Disease Control, Shenzhen 518000, China; 2 Department of Dermatology, Dermatology Hospital, Southern Medical University, Guangzhou 510091, China
    * Co-first author
  • Online:2024-06-15 Published:2024-05-07

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摘要： 目的：明确溶质转运体家族蛋白SLC35E在多菌型（MB)与少菌型(PB)麻风皮损中的差异。方法：首先收集25例确诊为麻风感染患者的石蜡包埋皮损组织，其中多菌型麻风患者12例，少菌型13例，分析SLC35E1和SLC35E2B不同SNP位点的突变情况；同时收集12名健康对照者皮肤组织作为正常对照组，采用免疫组化染色法检测SLC35E1、SLC35E2B、CD68、S100在上述皮损及正常对照组中的表达差异，免疫荧光染色法检测CD68、S100与SLC35E1、SLC35E2B蛋白在皮损组织中的共表达情况。结果：对25例麻风组织样本进行生物信息学分析，SLC35E1的SNP位点rs202071873在麻风患者中的突变率为0％，SLC35E2B的3个SNP位点rs12729295、rs117820608、rs2072923在麻风患者中的突变率分别为100%、40%，100%。免疫组化染色显示SLC35E1、SLC35E2B、CD68、S100在麻风组织的真皮及表皮均有表达，其中CD68主要表达于麻风组织真皮，SLC35E1在真皮层的表达量与CD68基本一致，两者表达量基本呈正相关；S100在麻风患者表皮和真皮中的表达无显著差异，与SLC35E1、SLC35E2B表达量无明显相关性；与少菌型麻风相比，多菌型麻风患者SLC35E1在真皮层呈低表达（P=0.046）；与健康对照组相比，多菌型麻风患者SLC35E2B在表皮层呈低表达（p=0.004）；免疫荧光共染显示麻风组织皮损中CD68与SLC35E1、SLC35E2B蛋白共染均出现重叠结果，且与SLC35E1重叠荧光强度高于SLC35E2B；S100与SLC35E1、SLC35E2B蛋白共染未见重叠结果。结论：麻风患者皮损中可能存在SLC35E2B位点突变，SLC35E1的表达可预测多菌型（MB)与少菌型(PB)麻风在真皮层感染差异，SLC35E1、SLC35E2B可能通过影响巨噬细胞功能干扰机体对麻风分枝杆菌的清除，进而影响麻风的发病。

关键词: 免疫应答, 巨噬细胞, 麻风, SLC35E1, SLC35E2B, CD68, S100

Abstract: Objective: To determine the difference of solute transporter family protein SLC35E between multibacillary and paucibacterial leprosy lesions. Methods: First, paraffin-embedded skin tissues of 25 patients with leprosy were collected, including 12 of multibacillary leprosy and 13 of  paucibacillary leprosy. The mutations of different SNPS of SLC35E1 and SLC35E2B were analyzed. At the same time, skin tissues of 12 healthy controls were collected as normal control group. Immunohistochemical staining was used to detect the expression differences of SLC35E1, SLC35E2B, CD68 and S100 in the above lesions and normal control group. Immunofluorescence staining was used to detect the co-expression of CD68 and S100 with SLC35E1 and SLC35E2B proteins in skin lesions. Results: Bioinformatics analysis of 25 leprosy tissue samples showed that the mutation rate of SNP rs202071873 of SLC35E1 in leprosy patients was 0%. SLC35E2B three SNPS loci rs12729295, rs117820608, rs2072923 mutation rate in leprosy patients were 100%, 40%, 100%. Immunohistochemical staining showed that SLC35E1, SLC35E2B, CD68 and S100 were expressed in the dermis and epidermis of leprosy tissues, among which CD68 was mainly expressed in the dermis of leprosy tissues, and the expression of SLC35E1 in the dermis was basically consistent with that of CD68, and there was a positive correlation between them. There was no significant difference in the expression of S100 between epidermis and dermis of leprosy patients, and no significant correlation with the expression of SLC35E1 and SLC35E2B. Compared with paucibacillary leprosy patients, SLC35E1 of multibacillary leprosy patients in the dermis was lower expression (P=0.046). Compared with healthy controls, the SLC35E2B of multibacillary leprosy patients in the epidermal layer was lower expression (P=0.004). Immunofluorescence co-staining showed that CD68 overlapped with SLC35E1 and SLC35E2B proteins in leprosy lesions, and the overlap fluorescence intensity with SLC35E1 was higher than that with SLC35E2B. No overlapping results were observed when S100 was co-stained with SLC35E1 and SLC35E2B proteins. Conclusion: There may be mutations in the SLC35E2B locus in leprosy lesions of  patients, and the expression of SLC35E1 may predict the difference between MB and PB infection in dermis. SLC35E1 and SLC35E2B may interfere with the clearance of M. leprae by affecting the function of macrophages, thereby affecting the pathogenesis of leprosy.

Key words: immune response, macrophages, leprosy, SLC35E1, SLC35E2B, CD68, S100