中国麻风皮肤病杂志 ›› 2016, Vol. 32 ›› Issue (7): 405-409.

• 论著 • 上一篇    下一篇

HPV感染宫颈组织中14-3-3σ表达及启动子甲基化水平检测

齐淑贞1 ， 王少明2 ， 张瑞丽3 ， 周斌兵4 ， 尤志学4 ， 苏晓红1 ， 王千秋1   

  1. 1.中国医学科学院皮肤病医院，南京，210042 2.中国医学科学院肿瘤医院肿瘤研究所，北京，100021 3.南京医科大学附属无锡第二医院皮肤科，江苏无锡，214002 4.南京医科大学第一附属医院妇科，南京，210029
  • 出版日期:2016-07-15 发布日期:2018-12-28
  • 通讯作者: 王千秋, E-mail: wangqq@ncstdlc.org, 苏晓红, E-mail:suxh@ncstdlc.org

The expression of 14-3-3σ and the detection of methylation of 14-3-3σ gene promoter in the cervix with HPV infection

QI Shuzhen1, WANG Shaoming2, ZHANG Ruili3, ZHOU Binbing4, YOU Zhixue4, SU Xiaohong1 , WANG Qianqiu1   

  1. 1. Institute of Dermatology, Chinese Academy of Medical Sciences，Nanjing 210042, China; 2. Cancer Hospital Chinese Academy of Medical Sciences, Beijing 100021, China; 3. Department of Dermatology, Wuxi Second Affiliated Hospital of Nanjing Medical University, Wuxi NO.2 People’s Hospital, Wuxi 214002, China 4. Department of Gynecology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China
  • Online:2016-07-15 Published:2018-12-28
  • Contact: WANG Qianqiu, E-mail: wangqq@ncstdlc.org SU Xiaohong E-mail:suxh@ncstdlc.org

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摘要： 目的: 检测HPV感染宫颈组织中14-3-3σ启动子甲基化与14-3-3σmRNA的表达。方法：采用甲基化特异性PCR技术检测26例宫颈CIN3+宫颈原位鳞癌，39例宫颈尖锐湿疣及35例对照标本中14-3-3σ基因启动子区CpG岛甲基化状态，Q-PCR法检测14-3-3σmRNA表达，Western Blot检测14-3-3σ蛋白的表达。 结果：宫颈CIN3+宫颈原位鳞癌组中14-3-3σ基因启动子区CpG岛甲基化率为73.08%，明显高于对照组的22.86%，差异有统计学意义（P＜0.05）；尖锐湿疣组为17.94%与对照组比较，差异无统计学意义（P＞0.05）。宫颈CIN3+宫颈原位鳞癌组的14-3-3σmRNA及蛋白表达较对照组减少，宫颈尖锐湿疣组14-3-3σmRNA及蛋白表达较对照组增加。 结论： HPV感染宫颈CIN3+宫颈原位鳞癌组织中14-3-3σ表达减少可能与14-3-3σ启动子高甲基化相关。

关键词: 尖锐湿疣, 人乳头瘤病毒;宫颈上皮内瘤变;宫颈原位鳞癌;甲基化, 14-3-3σ

Abstract: Objective: To detect the level of methylation of 14-3-3σ gene promoter region, and the expression of 14-3-3σ mRNA and protein in cervix with HPV infection. Methods: The levels of methylation of 14-3-3σ gene promoter CPG island，and the expression of 14-3-3σ mRNA and protein were detected by methylation-specific PCR, Q-PCR and western-blot respectively in 39 cases with cervical condyloma accuminatum (CA), 35 controls and 26 cases with cervical intraepithelial neoplasia (CIN3) complicated with cervical squamous cell carcinoma (SCC). Results: The rate of the methylation of 14-3-3σ gene promoter CPG island in the samples of SCC was 73.08%, which was higher than that inof control group (22.86%), with a significant difference (P＜0.05). There was no significant difference inof the rate between the samples of CA (17.94%) and of controls (P＞0.05). The expression of 14-3-3σ mRNA and protein in the patients with CIN3 complicated with SCC was lower than that in controls (P<0.05). The expression of 14-3-3σ mRNA and protein in the patients with CA was higher than those in c ontrols (P<0.05). Conclusion: The reduced expression of 14-3-3σ in the patients with CIN3 complicated with SCC may be associated with the hypermethylation of 14-3-3σ gene promoter region.

Key words: condyloma accuminatum, HPV, CIN, Cervical squamous cell carcinoma, methylation, 14-3-3σ