中国麻风皮肤病杂志 ›› 2017, Vol. 33 ›› Issue (6): 328-331.

• 论著 • 上一篇    下一篇

扁平苔藓皮损中microRNA-155的表达及与Th17/Treg失衡的相关性

贺琪1，3  朱旋2，3  皮先明1，3  石全1，3  李家文4  胡枫5   

  1. 1湖北省中医院皮肤科，武汉，430061
    2湖北省中医院泌尿外科，武汉，430061
    3湖北省中医药研究院，武汉，430074
    4华中科技大学同济医学院附属协和医院皮肤科，武汉，430022
    5武汉市第一医院皮肤科，武汉，430030
  • 出版日期:2017-06-15 发布日期:2018-12-13
  • 通讯作者: 贺琪,E-mail: catty0004@163.com

Expression of microRNA-155 and the relationship with imbalance of Th17/Treg in the patients with lichen planus

HE Qi1，3, ZHU Xuan2，3, PI Xianming1，3, SHI Quan1，3, LI Jiawen4, HU Feng5.   

  1. 1. Department of Dermatology, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan 430061, China; 
    2. Department of Urology, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan 430061, China; 
    3. Hubei Province Academy of Traditional Chinese Medicine, Wuhan 430074, China; 
    4. Department of Dermatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China; 
    5. Department of Dermatology, Wuhan First Hospital, Wuhan 430030, China
  • Online:2017-06-15 Published:2018-12-13
  • Contact: HE Qi, E-mail: catty0004@163.com

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摘要： 目的：检测microRNA-155（miR-155）在皮肤扁平苔藓（CLP）中的表达，明确其与辅助性T细胞17 /调节性T细胞（Th17/Treg）失衡的相关性。方法：应用TaqMan探针法检测33例CLP患者和25名健康对照者外周血中miR-155，孤儿受体-γt（RORγt），白细胞介素-17A（IL-17A），叉状头/翼状螺旋转录因子P3（Foxp3）和IL-10的基因表达水平。结果：CLP中miR-155，RORγt、Foxp3和IL-17A 的表达较对照组显著升高，而IL-10的表达显著降低（均P＜0.05）；CLP组中，miR-155分别与RORγt、Foxp3和IL-17A的含量显著正相关（均P＜0.001），与IL-10的含量无明显相关性（P＞0.05）。结论：CLP中的miR-155过表达可能与Th17/Treg失衡有关。

关键词: microRNA-155, 扁平苔藓, 辅助性T细胞17, 调节性T细胞, 白细胞介素-17A

Abstract: Objective: To detect the expression level of microRNA-155 (miR-155) and to determine the relationship with the imbalance of T-helper 17 cell/regulatory T cell (Th17/Treg) in the patients with cutaneous lichen planus (CLP). Methods: The expression of miR-155, retinoid-acid receptor-related orphan receptor gamma (RORγt), interleukin-17A (IL-17A), forkhead box P3 (Foxp3) and IL-10 in peripheral blood of 33 patients with CLP and 25 normal controls were detected by TaqMan probe. Results: Compared with the normal controls, the expression levels of miR-155, RORγt, Foxp3 and IL-17A were higher and the level of IL-10 expression was lower in the CLP patients with CLP (P＜0.05). The level of miR-155 expression was positively correlated with the expression levels of of RORγt, Foxp3 and IL-17A in the patients with CLP (Ps＜0.001). There was no correlation between the expression level of miR-155 and IL-10. Conclusion: The miR-155-upregulation existed in the CLP patients may be associated with the imbalance of Th17/Treg.

Key words: microRNA-155, lichen planus, T-helper 17 cell, regulatory T cell, interleukin-17A