中国麻风皮肤病杂志 ›› 2019, Vol. 35 ›› Issue (9): 529-532.doi: 10.12144/zgmfskin201909529

• 论著 • 上一篇    下一篇

Th17相关细胞因子和趋化因子在天疱疮发病中的作用

叶珊珊  李莹洁  李卉  姚香君  王再兴   

  1. 安徽医科大学第一附属医院皮肤性病科，合肥，230000
  • 出版日期:2019-09-15 发布日期:2019-09-16
  • 通讯作者: 王再兴，E-mail:wzx2370@163.com

The role of Th17 related cytokines and chemokines in pemphigus

YE Shanshan, LI Yingjie, LI Hui, YAO Xiangjun, WANG Zaixing   

  1. Department of Dermatology and Venereology, The First Affiliated Hospital of Anhui Medical University, Anhui 230000, China
  • Online:2019-09-15 Published:2019-09-16
  • Contact: WANG Zaixing, E-mail: wzx2370@163.com

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摘要： 目的：探讨Th17相关细胞因子IL-6、IL-17、IL-21、IL-22、IL-23和趋化因子CXCL-8、CCL-5在天疱疮发病中的作用。方法：采用酶联免疫吸附试验（ELISA）检测天疱疮患者治疗前后、大疱性类天疱疮患者及健康对照者的Th17相关细胞因子和趋化因子水平，并分析天疱疮严重程度及其亚型中这些因子的表达水平。结果：共收集天疱疮患者31例，其中寻常型天疱疮18例，落叶型天疱疮7例，红斑型天疱疮6例。另选取明确诊断为大疱性类天疱疮初发患者15例及20名健康人作为对照。天疱疮组中Th17相关因子IL-6、IL-17、IL-21、IL-22、IL-23和趋化因子CXCL-8、CCL-5水平分别为(32.31±7.98)pg/mL,(17.00±4.53)pg/mL,(491.13±72.41)pg/mL,(150.91±23.32)pg/mL,(264.89±67.84)pg/mL,(27.91±10.79)pg/mL,(47.21±17.83)pg/mL明显高于健康对照组的(8.36±1.96)pg/mL,(8.41±1.36)pg/mL,(372.73±18.45)pg/mL,(93.78±9.47)pg/mL,(163.17±9.48)pg/mL,(8.89±4.13)pg/mL,(22.66±2.85)pg/mL，差异均有统计学意义（P＜0.001）；天疱疮患者治疗后，IL-6、IL-17、IL-21、IL-22、IL-23和趋化因子CXCL-8水平较治疗前明显下降（P＜0.001）。IL-21在天疱疮组（491.13±72.41）pg/mL与大疱性类天疱疮组(559.58±78.56)pg/mL中差异有统计学意义（P＜0.05），其余因子差异无统计学意义（均P＞0.05）；寻常型天疱疮、落叶型天疱疮及红斑型天疱疮患者的7种因子水平比较，差异均无统计学意义（均P＞0.05）；IL-17、IL-21、IL-22、IL-23、CCL-5与疾病严重程度有关（P＜0.05）。结论：Th17相关的细胞因子和趋化因子可能与天疱疮的发病和疾病严重程度有关。

关键词: 天疱疮, 辅助性T细胞17, 细胞因子, 趋化因子

Abstract: Objective: To investigate the role of Th17-associated cytokines IL-6, IL-17, IL-21, IL-22, IL-23 and chemokines CXCL-8 and CCL-5 in the pathogenesis of pemphigus. Methods: The levels of Th17 related factors were detected by ELISA in patients with pemphigus before and after treatment, patients with bullous pemphigoid and healthy human controls. The relationships  of the severity of pemphigus and  Th17-associated cytokines expression levels in various subtype were analyzed. Results: Thirty one patients with pemphigus were collected including 18 cases of pemphigus vulgaris, 7 cases of pemphigus foliaceus and 6 cases of pemphigus erythematous. And 15 patients with bullous pemphigoid and 20 healthy were taken as controls. The levels of IL-6, IL-17, IL-21, IL-22, IL-23 and chemokines CXCL-8 and CCL-5 in pemphigus group were (32.31±7.98)pg/mL, (17.00±4.53)pg/mL, (491.13±72.41)pg/mL, (150.91±23.32)pg/mL, (264.89±67.84)pg/mL, (27.91±10.79)pg/mL, (47.21±17.83)pg/mL, which were significantly higher than those in healthy controls (8.36±1.96)pg/mL, (8.41±1.36)pg/mL, (372.73±18.45)pg/mL, (93.78±9.47)pg/mL, (163.17±9.48)pg/mL, (8.89±4.13)pg/mL, (22.66±2.85)pg/mL, with significant differences (Ps<0.001). After the treatment of the pemphigus patients, the level of IL-6, IL-17, IL-21, IL-22, IL-23 and chemokines CXCL-8, except for CCL-5, decreased (P<0.001). There was a significant difference of the IL-21 level in pemphigus group (491.13±72.41)pg/mL and bullous pemphigoid group (559.58±78.56), (P<0.05), and there were no significant difference of other cytokines (Ps>0.05). There was no significant differences of all the cytokines in the patients with pemphigus vulgaris, pemphigus foliaceus, pemphigus erythematous (Ps＞0.05). There were correlations between the levels of IL-17, IL-21, IL-22, IL-23, CCL-5 and the severity of pemphigus (P<0.05).  Conclusion: Th17-associated cytokines and chemokines may be involved in the pathogenesis and associated with the severity of pemphigus.

Key words: pemphigus, Th17 cell, cytokine, chemokine