中国麻风皮肤病杂志 ›› 2018, Vol. 34 ›› Issue (5): 257-260“264.

• 论著 •    下一篇

131例神经系统疾病患者血清中抗BP180和抗BP230抗体的检测

王亚如1,2，李洪军3，许德志4，王建文2，周桂芝2，杨宝琦2*   

  1. 1.济南大学山东省医学科学院医学与生命科学学院，山东 济南，250062 2.山东省医学科学院山东省皮肤病性病防治研究所，山东 济南，250022 3.泰安市中心医院神经内科，山东 泰安，271000 4. 济南市第五人民医院神经外科，山东济南 250022
  • 出版日期:2018-05-15 发布日期:2018-12-10
  • 通讯作者: 杨宝琦，E-mail：baoqiyang@126.com

Anti-BP180 and 230 antibodies in 131 patients with neurological diseases

WANG Yaru, LI Hongjun, XU Dezhi, WANG Jianwen, ZHOU Guizhi, YANG Baoqi   

  1. 1.School of Medicine and Life Sciences, Jinan University -Shandong Academy of Medical Sciences, Jinan 250062, China; 2.Shandong Provincial Institute of Dermatology and Venereology, Shandong Academy of Medical Sciences, Jinan 250022, China; 3.Department of Neurology, Taian City Central Hospital, Taian, 271000,China; 4.Department of Neurosurgery, the 5th People’s Hospital of Jinan, Jinan,250022, China
  • Online:2018-05-15 Published:2018-12-10
  • Contact: YANG Baoqi. E-mail: baoqiyang@126.com

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摘要： 目的：检测神经系统疾病患者血清中抗BP180、抗BP230和抗基底膜带抗体的阳性率。方法：收集神经系统疾病患者和正常对照血清，采用酶联免疫吸附试验（ELISA）和间接免疫荧光（IIF）检测两组血清中抗BPl80NC16A抗体和BP230抗体水平。结果：共收集到131例神经系统疾病患者血清(脑卒中109例，脑肿瘤17例，其他神经系统疾病19例)和131例正常对照血清。病例组中抗BP180NC16A1阳性率为1.45%，低于对照组的3.05%，差异具有统计学差异（P = 0.009），病例组中抗BP230抗体阳性率5.34%与对照组（2.29%）比较，差异无统计学意义。IIF检测抗基底膜带抗体结果均为阴性。结论：抗BP180NC16A抗体在神经系统疾病患者中有较高的阳性率，可能与神经系统疾病患者合并大疱性类天疱疮相关。

关键词: 大疱性类天疱疮, 神经系统疾病, BP180, BP230, 基底膜带

Abstract: Objective: To detect the level of anti-BP180 and 230 antibodies in the patients with neurological diseases (ND) , but without BP. Methods: The serum and clinical data of the patients with ND and healthy controls were collected. The levels of anti-BP 180, 230 antibodies and anti-basement membrane antibodies were detected by enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescence (IIF) respectively. Results: One hundred and thirty-one patients with ND (109 with cerebral apoplexy, 17 with brain tumors and 19 with other neurological disease) and 131 healthy controls were enrolled. Fifteen patients (11.45%) and 4 controls (3.05%) were positive for anti-BP180, NC16A antibodies, with a significant difference of P=0.009. Seven patients (5.34%) and 3 controls (2.29%) were positive for anti-BP 230 antibodies, with no significant difference (P=0.20). The anti-basement membrane antibodies were negative in the patients and healthy controls. Conclusion: The positivity rate of anti-BP180 antibodies was higher in the patients with neurological diseases, which may be associated with the pathogenesis of ND complicated with BP.

Key words: bullous pemphigoid, neurological disease, BP180, BP230, basement membrane zone