中国麻风皮肤病杂志 ›› 2021, Vol. 37 ›› Issue (6): 356-359.doi: 10.12144/zgmfskin202106356

• 论著 • 上一篇    下一篇

梅毒血清固定患者外周血Th1，Th2，Treg和Th17相关细胞因子检测

温晶,李珍,赵伟,李富容,侯建玲,李中伟,郑荣涛,田洪青   

  1. 山东第一医科大学附属皮肤病医院（山东省皮肤病医院，山东省皮肤病性病防治研究所），山东济南，250022
  • 出版日期:2021-06-15 发布日期:2021-05-27
  • 通讯作者: 田洪青，E-mail: tianhq2006@126.com

Analysis of Th1 cells, Th2 cells, Treg cells and Th17 cells related cytokines in peripheral blood of the serofast syphilis patients

WEN Jing, LI Zhen, ZHAO Wei, LI Furong, HOU Jianling, LI Zhongwei, ZHENH Rongtao, TIAN Hongqing   

  1. Shandong Provincial Hospital for Skin Diseases & Shandong Provincial Institute of Dermatology and Venereology, Shandong First Medical University, Jinan 250022, China
  • Online:2021-06-15 Published:2021-05-27
  • Contact: TIAN Hongqing, E-mail: tianhq2006@126.com

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摘要： 目的：探讨梅毒血清固定患者Th1，Th2，Treg和Th17相关细胞因子与梅毒血清固定的关系。方法：采用酶联免疫吸附试验法(ELISA)分别对不同组（梅毒血清固定组、新发梅毒组、梅毒治疗后转阴组和正常对照组）人群外周血进行Th1、Th2、Treg和Th17相关细胞因子（IFN-γ、IL-2、IL-12、IL-4、IL-10、IL-6、IL-17、IL-22、TGF-β的）检测，并进行相关性分析。
结果：与新发梅毒组、梅毒治疗后转阴组以及对照组比较，梅毒血清固定组IFN-γ、IL-2和IL-12水平均降低，差异均有统计学意义（均P<0.05）；但IL-4、IL-10及TGF-β水平均升高，差异均有统计学意义（均P<0.05）。血清固定组IL-17水平低于新发梅毒组和转阴组（均P<0.05）；IL-22低于新发梅毒组（P<0.05）；IL-6高于对照组（P<0.05）。结论：梅毒血清固定患者可能同时存在Th1细胞/Th2细胞，Treg细胞/Th17细胞的失衡，从而影响患者体内整体免疫应答水平。

关键词: 梅毒血清固定, Th1细胞, Th2细胞, Treg细胞, Th17细胞, 细胞因子

Abstract: Objective: To investigate the relationship between Th1，Th2，Treg and Th17 related cytokines and serofast syphilitic. Methods: The levels of Th1，Th2，Treg and Th17-related cytokines (IFN-γ, IL-2, IL-12, IL-4, IL-10, IL-6, IL-17, IL-22, TGF-β) in peripheral blood of different groups (serofast syphilis, syphilis, syphilis seroconvert and control) were detected by ElISA. Results: The levels of IFN-γ , IL-2 and IL-12 were lower and the levels of IL-4, IL-10 and TGF-β were higher in the Syphilis serofast group than in the syphilis group, syphilis seroconvert group and control group, with significant differences (Ps<0.05). The level of IL-17 in serofast syphilis group was significantly lower than that in syphilis group and control group (Ps<0.05), while IL-22 in serofast syphilis group was significantly lower than that in syphilis group (P<0.05), IL-6 in syphilis serofast group was significantly higher than that in control group (P<0.05). Conclusion: The imbalance of Th1，Th2，Treg and Th17 cells and TH17 cells may exist in the patients with serofast syphilis, which affects the whole immune response level of the patients.

Key words: syphilis serofast, Th1 cells, Th2 cells, Treg cells, Th17 cells, cytokines