中国麻风皮肤病杂志 ›› 2024, Vol. 40 ›› Issue (9): 624-629.doi: 10.12144/zgmfskin202409624

• 论著 • 上一篇    下一篇

青蒿油对小鼠痤疮模型及皮肤微生态的干预作用研究

黄虎1，2,杨志波3,常怀龙1，2,陶侃1，2,秦紫嫣1，2,郭莉莉1，2,何彰华4,毛必瑶4   

  1. 1上海上美化妆品股份有限公司全球研发中心，上海，200065；2上海昆药生物科技有限公司，上海，200000；3湖南中医药大学第二附属医院皮肤科，湖南长沙，410005；4湖南讴睿生物科技有限公司，湖南长沙，410205
  • 出版日期:2024-09-15 发布日期:2024-08-14

Intervention effect of Artemisia Naphta oil on mouse acne model and skin microecology

HUANG Hu1,2, YANG Zhibo3, CHANG Huailong1,2, TAO Kan1,2, QIN Ziyan1,2, GUO Lili1,2, HE Zhanghua4, MAO Biyao4   

  1. 1 Shanghai Shangmei Cosmetics Co., LTD. Global Research and development center, Shanghai 200065, China; 2 Shanghai KPC Biotechnology Co., LTD, Shanghai 200000, China; 3 Department of Dermatology, the Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha 410005, China; 4 Hunan Ourui Biotechnology Co., LTD, Changsha 410205, China
  • Online:2024-09-15 Published:2024-08-14

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摘要： 目的：评价青蒿油（artemisia naphta oil, AN）对痤疮丙酸杆菌（P. acnes）诱导的小鼠痤疮模型的疗效及皮肤微生态的影响。方法：将48只小鼠分为正常组、模型组、Base组、不同浓度AN干预组（0.5%、1.0%、1.5%、3.0%、5.0% AN组），每组6只。P. acnes注射耳朵皮内构建痤疮模型，小鼠右耳出现红肿后，Base组涂抹Base水对照干预，其余给予不同浓度的AN干预，每次涂抹1 mL，每天1次，17天后观察小鼠耳部厚度，HE染色观察皮肤病理，16S rRNA测序分析皮肤微生态变化，Western blot检测IL-6、IL-1β和TNF-α含量和TLR2、TLR4、P65、p-P65蛋白表达。结果：模型组小鼠耳部出现红肿、表皮层增厚、真皮层炎症细胞浸润，耳部菌群丰度和多样性降低，其中葡萄球菌、粘质沙门氏菌菌群丰度显著增加，双歧杆菌属、棒状杆菌属、Atopostipes菌群丰度显著下调；IL-6、IL-1β、TNF-α含量和TLR2、TLR4、p-P65/P65蛋白表达显著上升。Base组小鼠TLR2、TLR4、p-P65蛋白表达水平无明显改变。不同浓度AN处理后的小鼠耳部红肿、病理损伤减轻，耳部菌群丰度和多样性改善，葡萄球菌、粘质沙门氏菌显著下调，双歧杆菌属、棒状杆菌属、Atopostipes菌群丰度显著上调，且IL-6、IL-1β、TNF-α含量和TLR2、TLR4、p-P65/P65蛋白表达显著下降。结论：AN可降低促炎因子分泌，减轻皮肤炎症反应，增加皮肤有益菌的丰度，抑制病原菌生长，恢复皮肤微生态平衡，有效改善痤疮炎症样皮损变化。

关键词: 青蒿油, 痤疮, 皮肤微生态, 炎症

Abstract: Objective: To investagate the intervention effect of artemisia naphta oil (AN) on mouse acne model and skin microecology induced by propionibacterium acnes (P. acnes). Methods: 48 mice were divided into normal group, model group, base group, at various concentrations of AN intervention groups (0.5%, 1.0%, 1.5%, 3.0% and 5.0% AN groups), 6 mice in each group. P. acnes was injected into the ear skin to establish acne models. After the right ear appeared redness and swelling, the Base group was treated with Base water, and the rest of the mice were treated with different concentrations of AN, 1 mL each time, once a day. After 17 days, the ear thickness of the mice was observed, the skin pathology was observed by HE staining, and the skin microecological changes were analyzed by 16S rRNA sequencing. The levels of IL-6, IL-1β and TNF-α and the protein expressions of TLR2, TLR4, P65 and p-P65 were detected by Western blot. Results: Compared to the mice in the normal group, the ears of the mice in the model group were red and swollen, the epidermis was thickened, and the abundance and diversity of microbiota were decreased, among which the abundance of Staphylococcus and Serratia was increased significantly, the abundance of Bifidobacteria, Corynebacterium and Atopostipes was reduced，and the contents of IL-6, IL-1β, TNF-α,TLR2, TLR4 and p-P65/P65protein increased significantly. There was no significant change in the expression of TLR2, TLR4 and p-P65 protein in the Base group. Compared to the model group, after treated with various concentrations of AN, the ear redness and pathological damage of the mice in the model group were gradually reduced, the abundance and diversity of ear microbiota were improved, Staphylococcus and Serratia were significantly downregulated, Bifidobacteria, Corynebacterium and Atopostipes were significantly up-regulated, and IL-6, IL-1β, TNF-α, TLR2, TLR4, p-P65/P65 protein expression significantly increased. Conclusion: AN can reduce the secretion of proinflammatory cytokines, relieve the skin inflammation, restore the balance of skin microecology, and effectively improve the changes of acne inflammatory skin lesions.

Key words: artemisia naphta oil, acne, skin microbiome, inflammation